Related Experiment Video
Updated: Jul 2, 2025
Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models
Published on: June 16, 2022
Modeling Acute Myeloid Leukemia Using StarPEG-Heparin Hydrogels
P Lewen Holloway1, Akhilandeshwari Ravichandran1, Julien Clegg1,2
1School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Kelvin Grove, QLD, Australia.
Abstract:
Biomimetic semi-synthetic hydrogels formed from a combination of star-shaped poly(ethylene glycol) (starPEG) and the glycosaminoglycan, heparin, allows for the three-dimensional (3D) culture of various cells and tissues. In this chapter, we describe methods for the use of starPEG-heparin hydrogels to cultivate primary and immortalized human acute myeloid leukemia (AML) cells. The resulting 3D culture models allow for the study of AML development and response to chemotherapeutic agents.
More Related Videos
10:32Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Published on: May 19, 2023
10:44Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study
Published on: September 22, 2023