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MicroRNAs Associated with Keloids Identified by Microarray Analysis and In Vitro Experiments.

Sichao Chen1, Wenchao Zhang1, Zenan Xia1

  • 1Department of Plastic and Reconstructive Surgery, Peking Union Medical college Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Molecular Biotechnology
|February 23, 2024
PubMed
Summary
This summary is machine-generated.

Five microRNAs (miRNAs) are upregulated in keloid tissue, playing key roles in keloid pathogenesis. These miRNAs, including hsa-miR-127-3p, represent potential therapeutic targets for keloid treatment.

Keywords:
EpigeneticsKeloidMicroRNAMicroarrayWGCNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • Dermatology

Background:

  • MicroRNAs (miRNAs) are critical regulators of gene expression.
  • Keloids are abnormal scars resulting from dysregulated cellular processes.
  • Identifying specific miRNAs involved in keloid development is essential for therapeutic advancements.

Purpose of the Study:

  • To identify and validate key microRNAs (miRNAs) associated with keloid pathogenesis.
  • To explore potential therapeutic molecular targets for keloid treatment.
  • To elucidate the regulatory pathways influenced by identified miRNAs in keloids.

Main Methods:

  • Weighted gene co-expression network analysis (WGCNA) on GSE113620 dataset.
  • Microarray analysis and quantitative real-time PCR (qRT-PCR) for miRNA expression validation.
  • Bioinformatic analyses including Gene Ontology (GO) and KEGG pathway enrichment analysis for target gene prediction.

Main Results:

  • The blue module from WGCNA showed the highest correlation with keloids.
  • Five hub miRNAs (hsa-miR-127-3p, hsa-miR-214-3p, hsa-miR-155-5p, hsa-miR-409-5p, hsa-miR-542-5p) were identified.
  • These five miRNAs were significantly upregulated in keloid tissues.
  • Target genes were enriched in gene transcription, protein phosphorylation, MAPK cascade, and PI3K-AKT signaling pathways.

Conclusions:

  • The identified five miRNAs (hsa-miR-127-3p, hsa-miR-155-5p, hsa-miR-214-3p, hsa-miR-409-5p, hsa-miR-542-5p) are crucial in keloid pathogenesis.
  • These miRNAs represent promising therapeutic targets for keloid treatment.
  • The findings highlight the role of these miRNAs in regulating key cellular pathways involved in keloid formation.