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Updated: May 4, 2026

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Published on: June 12, 2020

Modeling embryo-endometrial interface recapitulating human embryo implantation.

Shun Shibata^1,2, Shun Endo^1,3, Luis A E Nagai⁴

¹Department of Informative Genetics, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan.

Science Advances

|February 23, 2024

Summary

Researchers created hormone-responsive endometrial organoids (AO-EMO) to model early human pregnancy. This 3D system successfully recapitulates key embryo implantation stages and feto-maternal interactions, advancing reproductive medicine research.

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Last Updated: May 4, 2026

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

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Published on: June 12, 2020

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

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Author Spotlight: In Vitro Endometrial Models to Study Epithelial-Stroma and Embryo Interactions

Author Spotlight: In Vitro Endometrial Models to Study Epithelial-Stroma and Embryo Interactions

Published on: November 8, 2024

Area of Science:

Reproductive Biology
Developmental Biology
Stem Cell Biology

Background:

Human embryo implantation is crucial for pregnancy initiation but poorly understood.
Existing models lack the complexity to fully replicate the in vivo endometrial environment.

Purpose of the Study:

To develop a novel 3D model system that accurately mimics early human embryo implantation.
To investigate the intricate feto-maternal interactions during the implantation process.

Main Methods:

Development of hormone-responsive apical-out endometrial organoids (AO-EMO) with native tissue architecture.
Coculture of AO-EMO with human embryonic stem cell-derived blastoids to form a 3D feto-maternal assembloid.
Validation of syncytiotrophoblast invasion and fusion with endometrial stromal cells using human blastocysts.

Main Results:

The AO-EMO model successfully recapitulates key implantation stages: apposition, adhesion, and invasion.
Syncytial cells derived from blastoids were observed to invade and fuse with endometrial stromal cells within the organoid model.
The model demonstrated validated fusion of syncytiotrophoblasts and stromal cells, mirroring human blastocyst interactions.

Conclusions:

The developed 3D feto-maternal assembloid system provides a robust platform for studying human embryo implantation.
This model offers unprecedented insights into early feto-maternal interactions, crucial for understanding reproductive health.
The findings pave the way for advancements in reproductive medicine and infertility research.