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Ca2+-selective microelectrodes.

D Ammann

    Cell Calcium
    |April 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Calcium (Ca2+) selective microelectrodes using ETH 1001 enable precise intracellular measurements of Ca2+ levels. These electrodes offer a detection limit of 10(-8) to 10(-7) M, providing localized cellular activity data.

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    Area of Science:

    • * Physiology
    • * Biophysics
    • * Analytical Chemistry

    Background:

    • * Accurate intracellular calcium (Ca2+) measurements are crucial for understanding cellular functions.
    • * Previous methods faced limitations in sensitivity and spatial resolution for dynamic Ca2+ monitoring.

    Purpose of the Study:

    • * To evaluate the efficacy of Ca2+-selective microelectrodes utilizing the ETH 1001 neutral carrier for intracellular Ca2+ quantification.
    • * To determine the detection limits and response times of these microelectrodes in biological systems.

    Main Methods:

    • * Development and application of Ca2+-selective microelectrodes with tip diameters >0.3 micron, incorporating the ETH 1001 ionophore.
    • * Quantitative measurements of intracellular resting Ca2+-activities and slowly changing Ca2+-levels.

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  • * Simultaneous recording of cellular electrical parameters (membrane potential, resistance) and other ion activities using multi-barrelled electrodes.

    • Main Results:

    • * Microelectrodes demonstrated a detection limit between 10(-8) and 10(-7) M Ca2+ within an intracellular environment.
    • * Response times were in the order of seconds, suitable for monitoring slow Ca2+ level changes.
    • * Localized Ca2+ determination was achieved, potentially indicating non-averaged cytosolic activity.

    Conclusions:

    • * ETH 1001-based Ca2+-selective microelectrodes provide a reliable tool for quantitative intracellular Ca2+ measurements.
    • * The technique allows for simultaneous assessment of electrical cell properties and localized Ca2+ dynamics.
    • * These microelectrodes offer improved detection limits and spatial resolution for studying cellular Ca2+ signaling.