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Amplification-free quantitative detection of genomic DNA using lateral flow strips for milk authentication
Nan Wang1, Juan Zhang1, Bin Xiao1
1Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Biosensors & Bioelectronics
|February 23, 2024
Summary
A new DNA hybridization method, LAMP-Like Hybridization (HybLAMP-Like), enables sensitive detection of cow's milk in dairy products. This nucleic acid quantification technique simplifies testing and accurately identifies adulteration without amplification.
Area of Science:
- Food Science
- Analytical Chemistry
- Biotechnology
Background:
- Globalization increases food fraud complexity.
- Current detection methods are lab-based, complex, and lack quantitative accuracy.
- Accurate detection of dairy product adulteration is crucial.
Purpose of the Study:
- To develop a novel, simplified nucleic acid quantification method for dairy products.
- To detect cow's milk adulteration using lateral flow strips (LFS).
- To establish an efficient and accurate quantitative detection technique.
Main Methods:
- Designed single-stranded DNA (ssDNA) probes targeting species-specific mitochondrial genes.
- Developed a new DNA hybridization method: LAMP-Like Hybridization (HybLAMP-Like).
- Utilized bst enzyme for probe-template binding, eliminating nucleic acid amplification.
Main Results:
- Achieved visual detection sensitivity down to 12.5 ng and reader-based detection down to 3.125 ng.
- Visually detected as low as 1% cow's milk in adulterated dairy products.
- Enabled precise quantitative analysis of milk adulteration with a portable LFS reader.
Conclusions:
- HybLAMP-Like offers a simplified, sensitive, and accurate method for dairy product analysis.
- The technique addresses limitations of traditional laboratory-based testing.
- This method enhances food safety by enabling efficient detection of adulteration.
