Ratiometric electrochemical immunosensor for the detection of CA199 based on the ratios of NiCo@Fc-MWCNTs-LDH and 3D-rGOF@Ag/Au complexes

Feifan Qi ¹, Mengdie Wu ², Simin Liu ¹

⁰Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Health Science and Engineering, University of Shanghai for science and technology, Shanghai, 200093, China.

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February 23, 2024

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Summary

A novel ratiometric electrochemical immunosensor was developed for sensitive detection of carbohydrate antigen 199 (CA199), a key pancreatic cancer marker. This assay accurately differentiates pancreatic cancer patients from healthy individuals using human plasma.

Area Of Science

Electrochemistry
Biosensing
Nanomaterials

Background

Carbohydrate antigen 199 (CA199) is a sensitive marker for pancreatic cancer, but developing highly sensitive detection assays remains challenging.
Electrochemical immunosensors offer potential for sensitive and quantitative biomarker detection.
Novel nanomaterials are crucial for enhancing sensor performance, including surface area and conductivity.

Purpose Of The Study

To develop a highly sensitive ratiometric electrochemical immunosensor for the quantitative analysis of CA199.
To utilize NiCo@Fc-MWCNTs-LDH and 3D-rGOF@Ag/Au nanomaterials for improved sensor performance.
To validate the immunosensor's accuracy in detecting CA199 in human plasma for pancreatic cancer diagnosis.

Main Methods

Fabrication of a ratiometric electrochemical immunosensor using NiCo@Fc-MWCNTs-LDH as the electrode and 3D-rGOF@Ag/Au as the label.
Utilizing the ratio of two distinct electrochemical signals (Ag and Fc-COOH) for quantitative CA199 detection.
Characterization of nanomaterial properties, including surface area, porosity, and conductivity.

Main Results

The immunosensor demonstrated excellent performance with a wide detection range (0.0001–10 U/mL) and a low detection limit (5.55 × 10⁻⁴ U/mL).
The ratio of electrochemical signals showed a linear relationship with CA199 concentration on a logarithmic scale.
The sensor exhibited high specificity and accuracy in detecting CA199 in human plasma, distinguishing pancreatic cancer patients from healthy individuals.

Conclusions

The developed ratiometric electrochemical immunosensor provides a highly sensitive and accurate method for CA199 detection.
This approach offers a promising tool for early diagnosis of pancreatic cancer.
The strategy presents a novel platform for the ultrasensitive quantitative detection of other disease biomarkers.

Keywords:

Carbohydrate antigen199 Layered double hydroxides Ratiometric electrochemical immunosensor Reduction of graphene oxide