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Label-free, liquid crystal-based aptasensor for detecting carbendazim at picomolar levels
1Department of Chemistry, Gachon University, Seongnam-daero 1342, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Republic of Korea.
Food Chemistry
|February 23, 2024
Summary
This study introduces a novel liquid-crystal (LC) method for detecting carbendazim (CBZ) using aptamer interactions. The technique offers sensitive and straightforward quantification of CBZ in various samples.
Area of Science:
- Analytical Chemistry
- Materials Science
- Biotechnology
Background:
- Carbendazim (CBZ) is a widely used fungicide requiring sensitive detection methods.
- Existing detection techniques can be complex or lack sufficient sensitivity.
- Liquid crystals (LCs) offer unique optical properties exploitable for sensing applications.
Purpose of the Study:
- To develop a simple and sensitive liquid-crystal (LC)-based assay for carbendazim (CBZ) quantification.
- To leverage aptamer-specific recognition at the aqueous-LC interface for detection.
- To assess the method's performance in real-world sample matrices.
Main Methods:
- Utilized aptamer-cetyltrimethylammonium bromide (CTAB) interfacial interactions modulated by carbendazim (CBZ).
- Observed changes in LC ordering (homeotropic to tilted transition) based on CBZ concentration.
- Employed a liquid-crystal (LC) based detection platform for interfacial sensing.
Main Results:
- Achieved a highly sensitive carbendazim (CBZ) detection limit of 3.12 pM (0.000597 μg/L).
- Demonstrated a linear detection range from 0.05 nM to 5 nM for carbendazim (CBZ).
- Successfully quantified carbendazim (CBZ) in complex matrices including peach, soil, and tap water.
Conclusions:
- The developed liquid-crystal (LC)-based method provides a sensitive, simple, and effective approach for carbendazim (CBZ) detection.
- This aptamer-functionalized LC assay shows significant potential for environmental and food safety monitoring.
- The method's straightforward nature and high sensitivity make it a promising tool for carbendazim (CBZ) analysis.
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