Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

2.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.9K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

5.6K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.6K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
GPCR Desensitization01:12

GPCR Desensitization

6.0K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
6.0K
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026
Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026
Same author

Non-invasive assessment of inflammatory bowel disease activity using a DIA-derived stool peptidomic signature and machine learning.

Frontiers in molecular biosciences·2026
Same author

Machine Learning-Derived Predictive Risk Score for Prediabetes and Type 2 Diabetes Development in Parous Women.

Diabetes, metabolic syndrome and obesity : targets and therapy·2026
Same author

Regional Brain Age Deviations Reveal Divergent Developmental Pathways in Youth.

Biological psychiatry. Cognitive neuroscience and neuroimaging·2026
Same author

Microproteins in Human Physiology and Pathology.

Biochemistry·2026

Related Experiment Video

Updated: Jul 2, 2025

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.1K

Galectin-8 modulates human osteoclast activity partly through isoform-specific interactions.

Michèle Roy1, Léopold Mbous Nguimbus1, Papa Yaya Badiane1

  • 1Division of Rheumatology, Department of Medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, Canada.

Life Science Alliance
|February 23, 2024
PubMed
Summary

This study reveals galectin-8

More Related Videos

Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

24.2K
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

7.9K

Related Experiment Videos

Last Updated: Jul 2, 2025

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.1K
Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

24.2K
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

7.9K

Area of Science:

  • Osteoclast biology
  • Cell signaling
  • Molecular interactions

Background:

  • Alternative splicing of LGALS8 (galectin-8) in osteoclasts reduces long isoform expression.
  • Galectin-8's roles in cell-matrix interactions and danger recognition are known, but its function in osteoclasts was uncharacterized.
  • Osteoclast overactivity is linked to bone diseases.

Purpose of the Study:

  • To investigate the role of galectin-8 in human osteoclast function.
  • To determine the specific contributions of galectin-8 isoforms to osteoclast biology.
  • To identify galectin-8 interacting proteins in osteoclasts.

Main Methods:

  • Inhibition of galectin-8 expression in human osteoclasts.
  • Isoform-specific inhibition of galectin-8.
  • Liquid chromatography with tandem mass spectrometry (LC-MS/MS) proteomic analysis.
  • Confirmation of protein interactions in human osteoclasts.

Main Results:

  • Galectin-8 inhibition impacts bone resorption, osteoclast nuclearity, and mTORC1 signaling.
  • The short isoform of galectin-8 plays a primary role in bone resorption.
  • LC-MS/MS identified shared and short isoform-specific interacting proteins, including cell adhesion and lysosomal proteins.
  • Confirmed interactions with CLCN3, CLCN7, LAMP1, and LAMP2, which localize to secretory vesicles.

Conclusions:

  • Galectin-8 has direct roles in regulating osteoclast activity.
  • The short galectin-8 isoform is predominantly responsible for these effects.
  • Galectin-8 interacts with key proteins involved in osteoclast function and vesicle transport.