Probing the combining site of monoclonal IgA J539 using deoxyfluoro- and other galactosides as ligands

IgA J539 is a monoclonal anti beta-(1----6)-D-galactopyranan. Measurement of its affinity with a number of synthetic galactosyl oligosaccharides of that linkage type, in which one or more hydroxyl groups have been replaced by a fluorine atom, confirm the assignment of the relative binding strength of the subsites in the protein for the individual galactosyl residues of the antigen.