Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.3K
Fixation and Sectioning01:03

Fixation and Sectioning

4.3K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Nanoscale Spatial Organization of ARC High- and Low-Order Assemblies at Excitatory Synapses.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Magnetic resonance control of spin-correlated radical pair dynamics in vivo.

Nature·2026
Same author

Quantum spin resonance in engineered proteins for multimodal sensing.

Nature·2026
Same author

Quantitative optical nanoscopy of mitochondrial-derived vesicles in neurons classifies pre-peroxisomal and clearing organelles.

Nature communications·2026
Same author

All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.

Nature communications·2025

Related Experiment Video

Updated: Jul 2, 2025

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

10.4K

Super-sectioning with multi-sheet reversible saturable optical fluorescence transitions (RESOLFT) microscopy.

Andreas Bodén1, Dirk Ollech1, Andrew G York2

  • 1Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.

Nature Methods
|February 23, 2024
PubMed
Summary

This study introduces multi-sheet RESOLFT, a super-resolution imaging technique. It achieves rapid, high-resolution 4D biological imaging of subcellular structures in living cells.

More Related Videos

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

16.7K
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
12:28

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

9.5K

Related Experiment Videos

Last Updated: Jul 2, 2025

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

10.4K
Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

16.7K
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
12:28

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

9.5K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Light-sheet fluorescence microscopy enables rapid 4D biological imaging but struggles with subcellular resolution.
  • The diffraction limit of light and sheet width (>1 μm) hinder fine subcellular detail retrieval in living cells.

Purpose of the Study:

  • To develop a super-resolution imaging method for rapid volumetric imaging of subcellular structures.
  • To overcome the resolution limitations of conventional light-sheet microscopy for live-cell imaging.

Main Methods:

  • Utilized reversibly switchable fluorescent proteins (RSFPs) and patterned illumination for photoswitching.
  • Developed multi-sheet RESOLFT, creating multiple sub-diffraction limit emission sheets in parallel.
  • Achieved super-sectioning ability (<100 nm) at high recording speeds (1-2 Hz).

Main Results:

  • Demonstrated super-resolution imaging of subcellular structures with enhanced detail.
  • Achieved rapid volumetric imaging (1-2 Hz) with super-sectioning (<100 nm).
  • Showcased compatibility with various RSFPs and minimal switching cycle requirements.

Conclusions:

  • Multi-sheet RESOLFT provides a powerful tool for high-resolution 4D live-cell imaging.
  • The technique enables tracking of dynamic cellular processes like cell division and particle motion.
  • This method significantly advances the study of subcellular dynamics in complex biological systems.