Eleutherococcus divaricatus Fruits Decrease Hyaluronidase Activity in Blood Serum and Protect from Antioxidative...

Area of Science:

• Pharmacology
• Ethnomedicine
• Phytochemistry

Background:

• Fruits are vital dietary sources of bioactive compounds, particularly polyphenols, crucial for preventing lifestyle-related diseases.
• Traditional ethnomedicine extensively utilizes various fruits, necessitating scientific validation of their phytochemical profiles and biological activities.
• Eleutherococcus divaricatus fruits have a history of ethnomedicinal use, warranting investigation into their chemical constituents and pharmacological effects.

Purpose of the Study:

• To determine the chemical composition of a methanolic extract from Eleutherococcus divaricatus fruits.
• To evaluate the biological activities, including anti-hyaluronidase, anti-tyrosinase, and antioxidant properties, of the fruit extract.
• To provide preliminary scientific evidence supporting the traditional applications of Eleutherococcus divaricatus in managing inflammatory and immune-related conditions.

Main Methods:

• Phytochemical analysis to identify and quantify specific polyphenolic compounds.
• In vitro assays to assess anti-hyaluronidase activity against bovine and human serum enzymes.
• Evaluation of anti-tyrosinase activity and antioxidant capacity using DPPH, ABTS, FRAP, and Fe2+ ion chelation assays.

Main Results:

• Chlorogenic acid, protocatechuic acid, and eleutheroside E were identified in the methanolic extract.
• The extract exhibited weak anti-hyaluronidase activity against bovine enzymes but high activity against human serum hyaluronidase from leukemia patients.
• Significant antioxidant activity was observed against DPPH, ABTS, and superoxide radicals, along with positive results in FRAP and Fe2+ chelation assays.

Conclusions:

• The identified polyphenolic compounds and observed biological activities partially validate the traditional ethnomedicinal use of Eleutherococcus divaricatus fruits.
• The extract's potent inhibition of human serum hyaluronidase and antioxidant effects suggest potential therapeutic benefits in inflammatory and immune-related diseases.
• Further in vivo and ex vivo research involving a larger patient cohort is recommended to elucidate the mechanisms and confirm the efficacy of Eleutherococcus divaricatus.