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  11. Differential Photosensitivity Of Fibroblasts Obtained From Normal Skin And Hypertrophic Scar Tissues

Differential Photosensitivity of Fibroblasts Obtained from Normal Skin and Hypertrophic Scar Tissues

Junya Kusumoto1,2, Masaya Akashi2, Hiroto Terashi1

  • 1Department of Plastic Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.

International Journal of Molecular Sciences
|February 24, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Blue light (BL) exposure synchronizes circadian rhythms in normal skin cells but not in hypertrophic scar cells. This finding suggests BL may help prevent and treat scars.

Area of Science:

  • Cell biology
  • Dermatology
  • Chronobiology

Background:

  • The photoreceptive nature of normal skin versus abnormal scarring is not well understood.
  • Opsins, crucial photoreceptor proteins, are implicated in cellular responses to light.

Purpose of the Study:

  • To investigate photosensitivity in normal dermal fibroblasts (NDFs) and hypertrophic scar fibroblasts (HSFs).
  • To examine the role of opsins, particularly OPN3, in fibroblast responses to blue light (BL).
  • To determine the effect of BL on circadian rhythmicity and alpha-smooth muscle actin (αSMA) expression in NDFs and HSFs.

Main Methods:

  • Quantified opsin expression in NDFs and HSFs.
  • Exposed both cell types to BL after circadian rhythm disruption.
  • Measured changes in αSMA, PER2, and BMAL1 gene expression.
Keywords:
OPN3blue lighthuman skin fibroblasthypertrophic scar

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  • Utilized OPN3 knockdown in HSFs to assess its role.

    • Main Results:

    • Opsins, with OPN3 being most abundant, were expressed in both NDFs and HSFs.
    • BL induced circadian rhythm formation in NDFs but not in HSFs.
    • HSFs exhibited higher αSMA levels, which decreased with BL exposure, an effect inhibited by OPN3 knockdown.

    Conclusions:

    • BL directly synchronizes peripheral circadian rhythms in NDFs, but not HSFs.
    • OPN3-mediated BL exposure inhibits HSFs, suggesting a therapeutic potential.
    • BL may be applicable for preventing and treating hypertrophic scars and keloids.
    peripheral circadian rhythm
    αSMA