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Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation.

Yonghui Bu1, Ping Wang1, Siqi Li1

  • 1State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangdong 510642, China.

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Cysteine-rich secretory protein 3 (CRISP3) in boar semen positively correlates with fertility and litter size. CRISP3 also exhibits immunomodulatory functions, potentially improving reproductive outcomes by regulating immune responses.

Keywords:
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Area of Science:

  • Reproductive Biology
  • Immunology
  • Animal Science

Background:

  • Semen proteins are crucial for male reproductive performance and fertility assessment.
  • The specific role of cysteine-rich secretory protein 3 (CRISP3) in male reproduction is not well understood.

Purpose of the Study:

  • To investigate the function of CRISP3 in boar reproductive performance.
  • To explore the potential of CRISP3 as a biomarker for male fertility.

Main Methods:

  • Quantified CRISP3 protein content in boar semen.
  • Assessed the localization of CRISP3 in sperm and its changes during capacitation.
  • Evaluated the effect of recombinant CRISP3 on sperm parameters and inflammatory gene expression in vitro.
  • Utilized lipopolysaccharide (LPS)-induced RAW264.7 cells to study immunomodulatory effects.

Main Results:

  • CRISP3 protein levels showed a significant positive correlation with boar fertility, sow delivery rate, and litter size.
  • CRISP3 is highly expressed in the bulbourethral gland and seminal plasma, localizing to the sperm head and tail.
  • Recombinant CRISP3 did not impact sperm motility or cleavage rate but downregulated inflammatory factors (IL-1α, IL-1β, IL-6) in LPS-stimulated cells.

Conclusions:

  • Boar semen CRISP3 protein levels are positively associated with reproductive success.
  • CRISP3 possesses immunomodulatory properties, suggesting a role in regulating immune responses within the female reproductive tract.
  • CRISP3 may enhance reproductive performance through immune modulation.