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Endogenous digoxin-immunoactive factor in human subjects.

R Valdes

    Federation Proceedings
    |September 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Endogenous digoxin-like immunoactivity is found in various patient groups, potentially affecting digoxin test accuracy. Altered protein binding of this factor explains its detection in common digoxin immunoassays.

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    Area of Science:

    • Cardiology
    • Clinical Chemistry
    • Biochemistry

    Background:

    • Endogenous digoxin-like immunoactivity (EDLI) is present in human blood, amniotic fluid, urine, and serum.
    • EDLI levels increase during pregnancy and may be elevated in hypertensive pregnant women.
    • This activity cross-reacts with antibodies used in commercial digoxin immunoassays.

    Purpose of the Study:

    • To summarize findings on detecting EDLI in clinical populations.
    • To investigate how EDLI may compromise accurate digoxin measurements.
    • To present preliminary isolation and characterization of the immunoactive factor.

    Main Methods:

    • Review of recent findings on EDLI detection in specific patient groups.
    • Analysis of EDLI levels in pregnant versus normotensive women.

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  • Preliminary isolation and characterization of the endogenous factor.

    • Main Results:

    • EDLI detected in renal failure patients, newborns, and pregnant women.
    • EDLI levels correlate with gestational progress and potentially with hypertension in pregnancy.
    • The endogenous factor is water-soluble and reversibly protein-bound, with altered binding in certain conditions.

    Conclusions:

    • Altered protein binding of EDLI is a key factor in its detection by digoxin immunoassays.
    • Accurate digoxin measurement can be compromised in populations with altered EDLI levels.
    • Further characterization of the endogenous factor is necessary.