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Related Concept Videos

Metastasis02:30

Metastasis

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Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
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The Tumor Microenvironment02:17

The Tumor Microenvironment

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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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  9. Predictive And Prognostic Markers
  11. Human Breast Cancer Cell Lines Differentially Modulate Signaling From Distant Microenvironments, Which Reflects Their Metastatic Potential.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Human Breast Cancer Cell Lines Differentially Modulate Signaling From Distant Microenvironments, Which Reflects Their Metastatic Potential.

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A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer
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A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer

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Human Breast Cancer Cell Lines Differentially Modulate Signaling from Distant Microenvironments, Which Reflects Their Metastatic Potential.

Ramon Ocadiz-Ruiz1, Joseph T Decker2, Kate Griffin1

  • 1Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Cancers
|February 24, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Metastatic breast cancers alter distant tissues, influencing tumor cell behavior like epithelial-to-mesenchymal transition (EMT). A novel scaffold mimics these changes, predicting metastatic potential without organ biopsy.

Keywords:
EMTbreast cancerimplantmetastasis

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Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior
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Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior

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Area of Science:

  • Oncology
  • Cancer Biology
  • Microenvironment Research

Background:

  • Metastasis significantly worsens cancer prognosis.
  • Tumor cell metastasis depends on intrinsic cell traits and distant tissue conditioning.
  • Understanding systemic effects of primary tumors on metastatic sites is crucial.

Purpose of the Study:

  • To investigate systemic alterations in distant tissues induced by human breast cancer cell lines.
  • To determine the impact of these tissue alterations on tumor cell phenotype.
  • To evaluate an implantable scaffold as a surrogate for lung tissue in assessing systemic alterations and metastatic potential.

Main Methods:

  • Utilized three human breast cancer cell lines (MDA-MB-231, MCF7, T47) to study lung tissue alterations.
microenvironment
scaffold
  • Analyzed changes in tumor cell phenotype including epithelial-to-mesenchymal transition (EMT), proliferation, migration, and senescence.
  • Employed an implantable scaffold as a surrogate for lung tissue to assess tumor-induced systemic effects.

    • Main Results:

    • Breast cancer cell lines significantly altered the lung microenvironment.
    • Lung microenvironment induced varying degrees of EMT, reduced proliferation, increased transendothelial migration, and senescence in tumor cells.
    • The implantable scaffold was conditioned by primary tumors similarly to the lung, promoting a pro-EMT profile.
    • Scaffold conditioning distinguished the metastatic potential of different breast cancer cell lines.

    Conclusions:

    • Metastatic and non-metastatic breast cancers condition distant tissues differently, affecting tumor cell responses.
    • A surrogate tissue scaffold can predict breast cancer cell line metastatic potential.
    • This approach offers a method to assess metastatic potential without invasive biopsy of vital organs.