Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Identification Of Tissue Mirna Signatures For Pancreatic Ductal Adenocarcinoma

Identification of Tissue miRNA Signatures for Pancreatic Ductal Adenocarcinoma

Carlo Caputo1, Michela Falco1,2, Anna Grimaldi3

  • 1Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via L. De Crecchio 7, 80138 Naples, Italy.

Cancers
|February 24, 2024

Related Experiment Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.4K
Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
05:44

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

4.3K
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.8K

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers identified specific microRNAs (miRNAs) linked to pancreatic cancer progression. Upregulated miR-31 and miR-205 in high-grade pancreatic ductal adenocarcinoma (PDAC) correlate with poorer survival, suggesting their potential as diagnostic biomarkers.

Keywords:
biomarkersdiagnosismicroRNAspancreatic ductal adenocarcinoma

Related Experiment Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.4K
Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
05:44

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

4.3K
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.8K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer mortality with poor prognosis.
  • Current diagnostic and prognostic tools for PDAC are insufficient.
  • Novel biomarkers are urgently needed for early detection and patient stratification.

Purpose of the Study:

  • To investigate deregulated microRNAs (miRNAs) in pancreatic ductal adenocarcinoma (PDAC) tissues.
  • To identify potential miRNA biomarkers for histological grade and lymph node metastasis in PDAC.
  • To explore the prognostic significance and therapeutic potential of identified miRNAs in PDAC.

Main Methods:

  • miRNA expression profiling using comprehensive PCR array and RT-qPCR validation.
  • Statistical analysis of miRNA expression in relation to histological grade (G2 vs. G3) and lymph node status (N- vs. N+).
  • In silico analysis (OncomiR, KEGG pathways) and survival analysis using TCGA PDAC clinical samples.

Main Results:

  • miR-1-3p, miR-31-5p, and miR-205-5p were significantly upregulated in high-grade (G3) PDAC.
  • miR-518d-3p upregulation and miR-215-5p downregulation were observed in PDAC with lymph node metastases (N+).
  • Overexpression of miR-31 and miR-205 correlated with decreased survival in PDAC patients.

Conclusions:

  • miR-1-3p, miR-31-5p, and miR-205-5p show potential as clinical biomarkers for PDAC progression.
  • These miRNAs may play a role in regulating key pathways like Hippo signaling and adherens junction.
  • Further investigation is warranted to validate these miRNAs as therapeutic targets for PDAC.
prognosis