Utilizing Integrated UHPLC-Q-Exactive Orbitrap-MS, Multivariate Analysis, and Bioactive Evaluation to Distinguish between Wild and Cultivated Niudali (Millettia speciosa Champ.)
- Yuwei Zeng 1, Qing Yang 2, Binbin Huang 3, Ming Chen 4, Zichang Liang 4, Zhifeng Zhang 2, Jianguang Zhang 2,4
- Yuwei Zeng 1, Qing Yang 2, Binbin Huang 3
- 1School of Pharmacy, Sichuan College of Traditional Chinese Medicine, Mianyang 621000, China.
- 2Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu 610041, China.
- 3Qin Zhou Inspection and Testing Center, Qinzhou 535000, China.
- 4Qin Zhou Provincial Health School, Qinzhou 535000, China.
- 0School of Pharmacy, Sichuan College of Traditional Chinese Medicine, Mianyang 621000, China.
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View abstract on PubMed
Summary
This summary is machine-generated.Wild and cultivated Millettia speciosa Champ. (MSCP) show significant antioxidant and anti-fatigue properties. This study identified key chemical markers to distinguish between MSCP varieties, aiding quality control and economic use.
Area Of Science
- Phytochemistry
- Pharmacology
- Chemometrics
Background
- Millettia speciosa Champ. (MSCP) is valued for culinary and medicinal uses.
- Quality and bioactivity differences between wild and cultivated MSCP are largely unknown.
- Understanding these differences is crucial for optimizing MSCP utilization.
Purpose Of The Study
- To investigate and compare the chemical profiles of wild and cultivated MSCP.
- To identify chemical markers for differentiating MSCP varieties.
- To evaluate the antioxidant and anti-fatigue properties of MSCP genotypes.
Main Methods
- Sample collection from diverse regions in China.
- Chemical constituent analysis using UHPLC-Q-Exactive Orbitrap-MS.
- Multivariate statistical analysis (PCA, OPLS-DA) for marker identification.
Main Results
- 62 chemical components were identified or characterized.
- Nine compounds were identified as potential chemical markers for MSCP differentiation.
- Both wild and cultivated MSCP demonstrated significant antioxidant and anti-fatigue activities, with wild types showing slightly higher bioactivity.
Conclusions
- This study provides a robust method for MSCP identification and quality control.
- Identified chemical markers facilitate the distinction between wild and cultivated MSCP.
- The findings highlight the economic potential of both MSCP genotypes, supporting their use in chemistry and pharmacology.
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