Utilizing Integrated UHPLC-Q-Exactive Orbitrap-MS, Multivariate Analysis, and Bioactive Evaluation to Distinguish between Wild and Cultivated Niudali (Millettia speciosa Champ.)

  • 0School of Pharmacy, Sichuan College of Traditional Chinese Medicine, Mianyang 621000, China.

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Summary

This summary is machine-generated.

Wild and cultivated Millettia speciosa Champ. (MSCP) show significant antioxidant and anti-fatigue properties. This study identified key chemical markers to distinguish between MSCP varieties, aiding quality control and economic use.

Area Of Science

  • Phytochemistry
  • Pharmacology
  • Chemometrics

Background

  • Millettia speciosa Champ. (MSCP) is valued for culinary and medicinal uses.
  • Quality and bioactivity differences between wild and cultivated MSCP are largely unknown.
  • Understanding these differences is crucial for optimizing MSCP utilization.

Purpose Of The Study

  • To investigate and compare the chemical profiles of wild and cultivated MSCP.
  • To identify chemical markers for differentiating MSCP varieties.
  • To evaluate the antioxidant and anti-fatigue properties of MSCP genotypes.

Main Methods

  • Sample collection from diverse regions in China.
  • Chemical constituent analysis using UHPLC-Q-Exactive Orbitrap-MS.
  • Multivariate statistical analysis (PCA, OPLS-DA) for marker identification.

Main Results

  • 62 chemical components were identified or characterized.
  • Nine compounds were identified as potential chemical markers for MSCP differentiation.
  • Both wild and cultivated MSCP demonstrated significant antioxidant and anti-fatigue activities, with wild types showing slightly higher bioactivity.

Conclusions

  • This study provides a robust method for MSCP identification and quality control.
  • Identified chemical markers facilitate the distinction between wild and cultivated MSCP.
  • The findings highlight the economic potential of both MSCP genotypes, supporting their use in chemistry and pharmacology.