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Intestinal Effects of Filtered Alkalinized Water in Lean and Obese Zucker Rats.

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Area of Science:

  • Gastroenterology and Nutrition
  • Microbiome Research
  • Oxidative Stress and Inflammation Studies

Background:

  • The gut microbiome plays a crucial role in host metabolism, inflammation, and overall health.
  • Oxidative stress and inflammation are implicated in various metabolic disorders.
  • Investigating the impact of water composition on gut health is essential for understanding dietary influences.

Purpose of the Study:

  • To evaluate the effects of alkalinized filtered water on the intestinal environment and metabolic status in lean and obese Zucker rats.
  • To assess the impact on gut microbiome composition, intestinal mucosa, inflammation, and oxidative stress markers.
  • To determine if alkalinized water consumption influences weight gain and antioxidant capacity.

Main Methods:

  • Adult Zucker rats (lean and obese) were administered either tap water or filtered alkalinized tap water for 3 months.
  • Metabolic changes were assessed using indirect calorimetry.
  • Plasma antioxidant capacity and inflammatory mediators were measured.
  • Fecal microbial composition, intestinal histology (MUC2, F4/80), and oxidative protein modifications (4-HNE) were analyzed using immunofluorescence and immunohistochemistry.

Main Results:

  • Consumption of alkalinized filtered water led to improvements in intestinal microbiome composition and the state of the intestinal mucosa.
  • Significant reductions in both local and systemic inflammation and oxidative stress levels were observed.
  • Alkalinized water consumption aided in maintaining a better oxidative status in rats.
  • No significant differences were noted in weight gain or overall antioxidant capacity between groups.
  • Probiotic supplementation demonstrated a notable impact on the gut microbiome.

Conclusions:

  • Alkalinized filtered water consumption positively modulates the gut microbiome and intestinal mucosa health.
  • It effectively reduces local and systemic inflammation and oxidative stress, contributing to improved oxidative status.
  • These findings suggest that alkalinized water can be a beneficial dietary component for enhancing metabolic, oxidative, and inflammatory health.