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Updated: Jul 2, 2025
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Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
Published on: October 16, 2016
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LIGHT regulated gene expression in rheumatoid synovial fibroblasts
Koji Fukuda1, Yasushi Miura2,3, Toshihisa Maeda1
1Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.
Molecular Biology Reports
|February 24, 2024
Summary
Lymphotoxin-related inducible ligand (LIGHT) influences gene expression in rheumatoid arthritis fibroblast-like synoviocytes. This suggests LIGHT plays a role in cell differentiation and function within rheumatoid arthritis pathogenesis.
Area of Science:
- Immunology
- Molecular Biology
- Rheumatology
Background:
- Rheumatoid arthritis (RA) is an autoimmune disease causing synovial hyperplasia, leading to cartilage and bone destruction.
- Lymphotoxin-related inducible ligand (LIGHT) is implicated in RA pathogenesis.
Purpose of the Study:
- To investigate the role of LIGHT in regulating gene expression in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS).
Main Methods:
- Utilized cDNA microarray analysis to compare gene expression profiles.
- Stimulated RA-FLS with LIGHT and analyzed changes.
Main Results:
- Identified significant gene expression changes (P < 0.05, fold change ≥ 2.0) in response to LIGHT.
- Affected genes were primarily associated with glycoprotein metabolism, N-linked glycosylation, plasma membrane localization, and extracellular functions.
Conclusions:
- LIGHT signaling impacts the expression of genes crucial for cell differentiation and function in RA-FLS.
- These findings highlight a potential regulatory role for LIGHT in RA progression.
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