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Synthetic Biology

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Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
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Employing Aeroponic Systems for the Clonal Propagation of Cannabis

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Published on: December 1, 2021

Engineering cannabinoid production in Saccharomyces cerevisiae.

Christina Schmidt¹, Marco Aras¹, Oliver Kayser¹

¹Technical Biochemistry Laboratory, Faculty of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.

Biotechnology Journal

|February 25, 2024

Summary

This study demonstrates efficient production of key cannabinoids, like cannabigerolic acid, in yeast. This breakthrough offers a promising alternative to plant-based extraction for pharmaceutical applications.

Keywords:

Cannabigerol NphB biosynthesis metabolic engineering yeast

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Microarray Analysis for Saccharomyces cerevisiae

Microarray Analysis for Saccharomyces cerevisiae

Published on: April 7, 2011

Area of Science:

Biotechnology
Synthetic Biology
Metabolic Engineering

Background:

Phytocannabinoids possess valuable pharmacological properties but plant extraction faces limitations.
Current heterologous production systems for cannabinoids lack industrially viable titers.
Developing alternative production methods is crucial for meeting demand.

Purpose of the Study:

To establish a robust yeast system for producing cannabinoids from simple feedstocks.
To enhance cannabinoid production titers through protein engineering and metabolic pathway optimization.
To demonstrate the de novo biosynthesis of specific cannabinoids in a heterologous host.

Main Methods:

Engineered a yeast system utilizing the aromatic prenyltransferase NphB.
Optimized precursor (olivetolic acid) production to 56 mg/L.
Implemented cannabinoid synthase genes and utilized a fed-batch approach for enhanced production.

Main Results:

Achieved production of cannabigerolic acid and cannabigerol from glucose and hexanoic acid.
Significantly increased production via an ERG20WW-NphB fusion protein.
Demonstrated first-time heterologous biosynthesis of cannabichromenic acid in yeast.
Showcased pH-dependent product spectrum of vacuole-localized cannabinoid synthases.
Attained titers of 18.2 mg/L for cannabigerolic acid and 117 mg/L for olivetolic acid.

Conclusions:

Developed a highly efficient heterologous yeast platform for cannabinoid biosynthesis.
The engineered system offers a scalable and controllable alternative to plant extraction.
Future research can leverage this platform for producing a wider range of cannabinoids.