Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

372
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
372
IR Spectrometers01:25

IR Spectrometers

1.2K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.2K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

685
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
685
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

893
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
893
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

367
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
367
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

390
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
390

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial intelligence-assisted estimation of postmortem intervals in bacterially infected cadavers using pathological imaging across variable temperature conditions.

Science & justice : journal of the Forensic Science Society·2025
Same author

A continuous forensic spectral analysis study to identify antemortem and postmortem muscle contusions by chemometrics.

Journal of forensic and legal medicine·2025
Same author

Large-vocabulary forensic pathological analyses via prototypical cross-modal contrastive learning.

Nature communications·2025
Same author

Identification of bacterial infection types in decomposition stages at various temperatures using pathology images and artificial intelligence algorithms.

Journal of microbiological methods·2025
Same author

Accurate forensic identification of asphyxial deaths: Differentiating strangulation and drowning using ATR-FTIR spectroscopy.

Science & justice : journal of the Forensic Science Society·2025
Same author

New perspective: An in vitro study on inferring the post-mortem interval (PMI) of human skeletal muscle based on ATR-FTIR spectroscopy combined with machine learning.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same journal

EXPRESS: Deterministic Compressed Sensing in Time-Domain Spectroscopy.

Applied spectroscopy·2026
Same journal

EXPRESS: Multi-Parameter Wavelength Characterization of Array Spectrometers Under Near-Limit Sampling Conditions.

Applied spectroscopy·2026
Same journal

EXPRESS: A Validated Reference Database for Twentieth-Century Cd-Based Pigments: Integrated Structural and Compositional Characterization.

Applied spectroscopy·2026
Same journal

EXPRESS: Two-Trace Two-Dimensional (2T2D-COS) in the Analysis of Brain Tissue Sample Preparation Method.

Applied spectroscopy·2026
Same journal

EXPRESS: Simplified Protocol for Analyzing Polarization Properties of Scanning Tunneling Microscope (STM) Light Emission Spectra at an Oblique Angle.

Applied spectroscopy·2026
Same journal

EXPRESS: Monitoring a Polyurethane Synthesis by Fiber-Coupled Attenuated Total Reflection Fourier Transform Infrared Spectroscopy and Multivariate Curve Resolution-Alternating Least Squares.

Applied spectroscopy·2026
See all related articles

Related Experiment Video

Updated: Jul 2, 2025

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

737

Ante- and Post-Mortem Fracture Identification Protocol Based on Low- and High-Level Fusion Using Fourier Transform

Kai Yu1, Hao Wu1, Hongli Xiong2

  • 1Department of Forensic Pathology, College of Forensic Medicine, Xi'an Jiaotong University, Xi'an, China.

Applied Spectroscopy
|February 26, 2024
PubMed
Summary
This summary is machine-generated.

This study combines Fourier transform infrared (FT-IR) and Raman spectroscopy with high-level fusion (HLF) to accurately identify antemortem and postmortem fractures. The HLF model achieved 0.88 accuracy, showing promise for forensic analysis at various postmortem intervals (PMIs).

Keywords:
Data fusionFourier transform infrared spectroscopyRamanantemortem and postmortem fracturesforensic pathology

More Related Videos

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.2K
A Method to Estimate Cadaveric Femur Cortical Strains During Fracture Testing Using Digital Image Correlation
09:34

A Method to Estimate Cadaveric Femur Cortical Strains During Fracture Testing Using Digital Image Correlation

Published on: September 14, 2017

7.4K

Related Experiment Videos

Last Updated: Jul 2, 2025

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

737
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.2K
A Method to Estimate Cadaveric Femur Cortical Strains During Fracture Testing Using Digital Image Correlation
09:34

A Method to Estimate Cadaveric Femur Cortical Strains During Fracture Testing Using Digital Image Correlation

Published on: September 14, 2017

7.4K

Area of Science:

  • Forensic Science
  • Analytical Chemistry
  • Biomaterials Science

Background:

  • Distinguishing antemortem from postmortem fractures is crucial in forensic investigations.
  • Traditional methods may face limitations in accuracy and specificity, especially at different postmortem intervals (PMIs).
  • Spectroscopic techniques offer potential for objective fracture analysis.

Purpose of the Study:

  • To investigate the combined application of FT-IR and Raman spectroscopy for identifying antemortem and postmortem fractures.
  • To evaluate the efficacy of low-level fusion (LLF) and high-level fusion (HLF) chemometric strategies for spectral data analysis.
  • To determine the optimal fusion strategy and key spectral features for accurate fracture classification across varying PMIs.

Main Methods:

  • Utilized Fourier transform infrared (FT-IR) and Raman spectroscopy to analyze hard tissue samples.
  • Applied low-level fusion (LLF) and high-level fusion (HLF) chemometric approaches to combine spectral data.
  • Assessed model performance using cross-validation and test dataset accuracy; identified important spectral features via intersection fusion.

Main Results:

  • The high-level fusion (HLF) model demonstrated superior performance, achieving an accuracy of 0.88 in both cross-validation and test datasets.
  • Fusion models (HLF and LLF+HLF) significantly outperformed single-technique models (FT-IR or Raman alone).
  • Key spectral features contributing to accurate identification included amide I, PO43-, CH2 bands (FT-IR) and phenylalanine, CO32-, amide III bands (Raman).

Conclusions:

  • The combination of FT-IR and Raman spectroscopy with the HLF strategy provides a robust and accurate method for identifying antemortem and postmortem fractures.
  • This integrated approach shows significant potential for application in forensic science, particularly for determining fracture timing at different postmortem intervals.
  • The identified spectral biomarkers offer valuable insights into the chemical changes associated with fractures over time.