Airborne pathogens diffusion: A comparison between tracer gas and pigmented aerosols for indoor environment analysis
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Updated: Jul 29, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
Marco Puglia1, Filippo Ottani1, Nicolo' Morselli1
1Università di Modena e Reggio Emilia, Dipartimento di Ingegneria "Enzo Ferrari", Via Pietro Vivarelli, 10-41125, Modena, Italy.
Abstract:
The evaluation of airborne pathogens diffusion is a crucial practice in preventing airborne diseases like COVID-19, especially in indoor environments. Through this transmission route, pathogens can be carried by droplets, droplet nuclei and aerosols and be conveyed over long distances. Therefore, understanding their diffusion is vital for prevention and curbing disease transmission. There are different techniques used for this purpose, and one of the most common is the utilization of tracer gas, however, it has limitations such as the difference in size between the gas molecules and the respiratory droplets, as well as its incapability to take into account evaporation. For this reason, a new method for evaluating the diffusion of respiratory droplets has been developed. This approach involves the use of an ultrasonic emitter to release and disperse pigmented aerosols, and a colorimeter for the following quantitative evaluation. A comparison with the tracer gas technique has been carried out, showing for the pigmented aerosols methodology a response that is dependent on different relative humidity conditions, while there is no clear difference in the dispersion of tracer gas at high or low humidity.
Insights
A new method using pigmented aerosols effectively evaluates airborne droplet diffusion indoors, unlike traditional tracer gas methods. This technique is sensitive to humidity, offering a more accurate understanding of disease transmission.
Area of Science:
- Environmental Science
- Public Health
- Aerosol Science
Background:
- Airborne pathogen diffusion is critical for preventing diseases like COVID-19 in indoor settings.
- Current methods like tracer gas have limitations, including size differences with droplets and inability to account for evaporation.
- Understanding pathogen spread is vital for effective disease control strategies.
Purpose of the Study:
- To develop and evaluate a novel method for assessing respiratory droplet diffusion indoors.
- To compare the performance of the new method against the conventional tracer gas technique.
- To investigate the influence of relative humidity on airborne droplet diffusion.
Main Methods:
- Utilized an ultrasonic emitter to generate and disperse pigmented aerosols.
- Employed a colorimeter for quantitative evaluation of aerosol dispersion.
- Conducted comparative analysis with the established tracer gas diffusion method.
Main Results:
- The pigmented aerosol method demonstrated humidity-dependent responses in dispersion.
- Tracer gas dispersion showed no significant variation across different relative humidity levels.
- The new method provides a more nuanced understanding of droplet behavior compared to tracer gas.
Conclusions:
- Pigmented aerosols offer a more realistic assessment of airborne droplet diffusion, accounting for environmental factors like humidity.
- This novel approach overcomes limitations of tracer gas methods for indoor airborne disease transmission studies.
- The findings support improved strategies for controlling airborne pathogen spread in enclosed spaces.
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