Response surface methodology-based optimisation of chitin production and its antioxidant activity from...

- In this study, we focused on isolating fungi capable of producing extracellular chitin, a critical component of fungal cell walls. Aspergillus niger was chosen as the candidate, and we aimed to optimise chitin production. Initially, one variable at a time (OVAT) method was used to enhance chitin yield under the best fermentation conditions. Subsequently, the Plackett-Burman design was employed to identify the key medium components influencing chitin production. These factors were then fine-tuned using the Central Composite Design, resulting in the optimal concentrations of dipotassium hydrogen phosphate (0.7 mg/l), calcium chloride (0.5 mg/l), thymine hydrochloride (0.5 mg/l), and pH (4), as confirmed by ANOVA. The application of response surface methodology (RSM) led to a remarkable improvement in chitin yield, increasing it from 1.14 g/l to an impressive 4.42 g/l, a substantial 3.34-fold enhancement compared to unoptimized conditions. Additionally, we explored the antioxidant activity of the produced chitin, revealing its promising properties with a scavenging activity ranging from 32% to 55% at concentrations of 1-2 mg/ml, surpassing the control. In conclusion, our study successfully optimized chitin production from Aspergillus niger and demonstrated the remarkable antioxidant potential of the produced chitin, highlighting its significance in various applications.