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Versatile high-performance liquid chromatographic computer system for solvent delivery, gradient control and data

C M Grisham, F Marquard, P L Jørgensen

    Journal of Chromatography
    |October 4, 1985
    PubMed
    Summary

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    A custom interface for high-performance liquid chromatography (HPLC) systems was developed using a Commodore 64 computer. This cost-effective solution offers precise solvent delivery and data acquisition, rivaling commercial systems.

    Area of Science:

    • Analytical Chemistry
    • Chromatography

    Background:

    • Commercial high-performance liquid chromatography (HPLC) systems often involve expensive and inflexible solvent programmers and data acquisition systems.
    • There is a need for more accessible and customizable HPLC control solutions.

    Purpose of the Study:

    • To design and implement a cost-effective interface for HPLC systems using a personal computer.
    • To provide precise control over solvent delivery and chromatographic data acquisition.

    Main Methods:

    • A Commodore 64 personal computer was integrated with a custom interface.
    • The interface controlled two solvent delivery pumps for isocratic or gradient elution.
    • Analog-to-digital conversion was used for chromatographic data acquisition.
    • Data output options included a dot-matrix printer or chart recorder.

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    Main Results:

    • The system achieved computer-controlled flow-rates up to 10.0 ml/min with high accuracy.
    • Flow-rate accuracy was 0.0047 ml/min at 4.1 ml/min and 0.03 ml/min at 10 ml/min.
    • Software enabled data analysis, including peak elution times, areas, and baseline correction.
    • Chromatographic data and relevant parameters were stored on floppy disks.

    Conclusions:

    • The developed interface provides a viable, economical alternative to commercial HPLC systems.
    • The system offers reliable solvent delivery and data handling capabilities for chromatographic analysis.
    • This approach demonstrates the potential of personal computers in controlling sophisticated analytical instrumentation.