Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Novel Carbapenem, JDB/PQ-1-219, Has Potent Broad Spectrum Activity against Multi-Drug Resistant <i>Acinetobacter baumannii</i>.

ACS infectious diseases·2026

Same author

Chemiluminescence microscopy single cell imaging using esterase triggered 1,2-dioxetanes.

The Analyst·2026

Same author

Viscosity effects on the chemiluminescence emission of 1,2-dioxetanes in water.

Organic & biomolecular chemistry·2025

Same author

An economical fluorescent method for microplastic detection in soil samples.

Analytical methods : advancing methods and applications·2025

Same author

A silicon rhodamine 1,2-dioxetane chemiluminophore for <i>in vivo</i> near-infrared imaging.

Organic & biomolecular chemistry·2025

Same author

Multi-stage and multi-colour liquid crystal reflections using a chiral triptycene photoswitchable dopant.

Nature chemistry·2024

Search research articles

Related Experiment Video

Updated: Jul 2, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Development of a solid-supported light-triggered nitric oxide donor.

Maidileyvis Castro Cabello¹, Alexander R Lippert¹

¹Department of Chemistry, Southern Methodist University, Dallas, Texas 75275-0314.

Journal of Photochemistry and Photobiology. A, Chemistry

|February 26, 2024

Summary

This summary is machine-generated.

Researchers developed a solid-supported nitric oxide (NO) donor for photopharmacology. This new NO donor, attached to polymer beads, releases NO upon light exposure for targeted cellular delivery.

Keywords:

cellular delivery nitric oxide photocleavable donors solid-phase chemistry

More Related Videos

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Related Experiment Videos

Last Updated: Jul 2, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Area of Science:

Photochemistry
Biomedical Engineering
Materials Science

Background:

Nitric oxide (NO) signaling is crucial in biological processes.
Photocleavable NO donors are valuable for NO research and photopharmacology.
Current research focuses on improving NO release kinetics and efficiency.

Purpose of the Study:

To design and synthesize a novel solid-supported photocleavable NO donor.
To investigate the NO release characteristics and detection methods.
To evaluate the biocompatibility and cellular NO delivery efficiency of the donor.

Main Methods:

Synthesis of an N-nitroso photocleavable NO derivative.
Immobilization of the NO derivative onto TentaGel® polymer resin beads.
NO release triggered by 365 nm light, detected by fluorescence and Griess assay.

Main Results:

Successful synthesis of solid-supported photocleavable NO donor beads.
Demonstrated NO release upon UV illumination, quantifiable via fluorescence and Griess assay.
Confirmed biocompatibility with A549 cells and localized NO delivery to proximal cells.

Conclusions:

Solid-supported NO donors offer a promising platform for photopharmacological applications.
The developed beads enable controlled and localized nitric oxide release.
This technology facilitates targeted NO delivery for cellular studies and therapeutics.