Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation of 1° Amines: Azide Synthesis01:22

Preparation of 1° Amines: Azide Synthesis

3.9K
Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
3.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigations into linker effects of DNA-VHL ligand conjugates by multiplexed affinity measurements using focal molography.

RSC chemical biology·2026
Same author

Aggregation of DNA oligomers with a neutral polymer facilitates DNA solubilization in organic solvents for DNA-encoded chemistry.

Chemical science·2025
Same author

Design and synthesis of a chemically diverse, lead-like DNA-encoded library from sequential amide coupling.

RSC medicinal chemistry·2025
Same author

Advancing TET Inhibitor Development: From Structural Insights to Biological Evaluation.

ACS medicinal chemistry letters·2025
Same author

Discovery of AIC263282, a Hepatitis B Virus Capsid Assembly Modulator Ready for Candidate Profiling.

Journal of medicinal chemistry·2025
Same author

Oxime Linked Doxorubicin Glycoconjugates Improve the Specific Targeting of Glioblastoma in High-Grade Glioma Therapy.

ACS medicinal chemistry letters·2024

Related Experiment Video

Updated: Jul 2, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.1K

Diversifying DNA-Tagged Amines by Isocyanide Multicomponent Reactions for DNA-Encoded Library Synthesis.

Suzanne Willems1, Elena Detta1, Lorenzo Baldini2

  • 1Faculty of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn-Straße 6, 44227 Dortmund, Germany.

ACS Omega
|February 26, 2024
PubMed
Summary

This study explores isocyanide multicomponent reactions to diversify DNA-tagged amines for DNA-encoded library synthesis, yielding diverse lactam scaffolds.

More Related Videos

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.1K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.2K

Related Experiment Videos

Last Updated: Jul 2, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.1K
High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.1K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.2K

Area of Science:

  • Organic Chemistry
  • Medicinal Chemistry
  • Synthetic Chemistry

Background:

  • Amine-substituted building blocks are crucial in DNA-encoded library synthesis.
  • Efficient methods are needed to diversify these building blocks for broader scaffold access.

Purpose of the Study:

  • To explore isocyanide multicomponent reactions for diversifying DNA-tagged amines.
  • To synthesize diverse lactam scaffolds using novel reaction pathways.

Main Methods:

  • Investigated isocyanide multicomponent reactions, including the Ugi-azide reaction.
  • Explored Ugi-aza-Wittig and Ugi-4-center-3-component reactions for lactam synthesis.
  • Utilized solid support-coupled DNA-tagged amines and bifunctional carboxylic acids.

Main Results:

  • Achieved high yields and good substrate scope with the Ugi-azide reaction.
  • Successfully synthesized five-, six-, and seven-membered lactams.
  • Demonstrated access to structurally diverse scaffolds through these reactions.

Conclusions:

  • Isocyanide multicomponent reactions offer a powerful strategy for DNA-tagged amine diversification.
  • The developed methods provide efficient access to diverse lactam scaffolds for library synthesis.