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Related Concept Videos

RNA Structure01:19

RNA Structure

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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Transcription Initiation01:47

Transcription Initiation

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Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
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Updated: Jul 2, 2025

An Assay for Quantifying Protein-RNA Binding in Bacteria
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Structural insights into IMP2 dimerization and RNA binding.

Stephen Zorc1,2, Paola Munoz-Tello3, Timothy O'Leary2

  • 1Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL, USA.

Biorxiv : the Preprint Server for Biology
|February 26, 2024
PubMed
Summary
This summary is machine-generated.

Full-length Insulin-like Growth Factor 2 mRNA-Binding Protein 2 (IGF2BP2) primarily forms dimers. RNA binding alters its structure, creating new dimer interfaces, which is crucial for developing targeted cancer and metabolic disorder therapies.

Keywords:
HDX-MSIGF2BP2IMP2RNA binding protein (RBP)SAXS

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Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • Insulin-like Growth Factor 2 mRNA-Binding Protein 2 (IGF2BP2) is implicated in cancer and metabolic diseases.
  • Understanding full-length IGF2BP2 structure is vital for drug discovery, but currently lacking.

Purpose of the Study:

  • To elucidate the structural behavior of full-length IGF2BP2.
  • To investigate the impact of RNA binding on IGF2BP2 structure and oligomerization.

Main Methods:

  • Mass photometry
  • Hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS)
  • Small-angle X-ray scattering (SAXS)

Main Results:

  • Full-length IGF2BP2 predominantly forms dimers, with oligomerization sensitive to ionic strength and RNA.
  • SAXS data modeling suggests a head-to-tail dimer orientation via KH34 and RRM1 domains in the apo state.
  • RNA binding induces a pseudo-symmetric dimer with a distinct interface involving KH12 domains.

Conclusions:

  • This study provides the first structural insights into full-length IGF2BP2.
  • Findings reveal RNA-dependent structural rearrangements in IGF2BP2 oligomerization.
  • These insights may facilitate the development of novel IGF2BP2 inhibitors for therapeutic intervention.