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Updated: Jul 2, 2025

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
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Nuclear receptor interdomain communication is mediated by the hinge with ligand specificity

Saurov Hazarika1, Tracy Yu2, Arumay Biswas1

  • 1Department of Chemistry, Pennsylvania State University, University Park, PA, 16802, USA.

Biorxiv : the Preprint Server for Biology
|February 26, 2024
PubMed
Summary

Ligand binding to nuclear receptors like farnesoid X receptor (FXR) causes domain rearrangement. Computational modeling and assays reveal the hinge region is crucial for this interdomain communication.

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