Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Haemagglutinin transport mutants.

W Garten, K Kuroda, W Schuy

    Vaccine
    |September 1, 1985
    PubMed
    Summary

    Two temperature-sensitive mutants with defects in haemagglutinin transport were studied. Point mutations in the stem region of haemagglutinin were identified in both mutants.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Intravirion cohesion of matrix protein M1 with ribonucleocapsid is a prerequisite of influenza virus infectivity.

    Virology·2016
    Same author

    Influenza A virus proteins NS1 and hemagglutinin along with M2 are involved in stimulation of autophagy in infected cells.

    Journal of virology·2013
    Same author

    Transmission studies resume for avian flu.

    Science (New York, N.Y.)·2013
    Same author

    [Evolution and infection biology of new influenza A viruses with pandemic potential].

    Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·2013
    Same author

    Post-translational modifications in insect cells.

    Cytotechnology·2012
    Same author

    Pause on avian flu transmission research.

    Science (New York, N.Y.)·2012

    Area of Science:

    • Molecular biology
    • Cell biology
    • Virology

    Background:

    • Intracellular transport is crucial for protein function.
    • Haemagglutinin (HA) transport from the endoplasmic reticulum (ER) to the plasma membrane is essential for viral activity.
    • Temperature-sensitive (ts) mutants provide valuable tools for studying transport pathways.

    Purpose of the Study:

    • To investigate the molecular basis of temperature-sensitive defects in haemagglutinin (HA) intracellular transport.
    • To identify specific mutations responsible for impaired HA trafficking in ts1 and ts651 mutants.

    Main Methods:

    • Analysis of two temperature-sensitive mutants (ts1 and ts651) exhibiting defects in HA transport.
    • Nucleotide sequencing of the haemagglutinin gene from both mutants.

    Main Results:

    • Both ts1 and ts651 mutants displayed temperature-sensitive defects in HA transport from the rough endoplasmic reticulum (RER) to the plasma membrane.
    • Nucleotide sequencing identified two distinct point mutations within the stem region of the haemagglutinin molecule for each mutant.

    Conclusions:

    • The identified point mutations in the HA stem region are likely responsible for the observed temperature-sensitive transport defects.
    • These findings contribute to understanding the structure-function relationships governing HA trafficking and viral assembly.

    Related Experiment Videos