Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.4K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

732
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
732
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nationwide outcomes of cardiovascular surgery in patients with Parkinson disease.

JTCVS open·2026
Same author

Endothelial KLF4 depletion drives age-related neurovascular dysfunction and neuropsychiatric impairment.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Outcomes of comorbid mental health disorders in cardiovascular surgery.

Journal of thoracic disease·2026
Same author

Outcomes of Cardiovascular Surgery in Patients With Multiple Sclerosis.

JAMA surgery·2026
Same author

Healthcare utilization following hip fractures based on social vulnerability status in the US: an analysis of 2016-2020 nationwide readmissions data.

Archives of orthopaedic and trauma surgery·2026
Same author

Femoroacetabular Impingement Rabbit Model Induced by Exercise and Mechanical Stimulation.

The American journal of sports medicine·2026

Related Experiment Video

Updated: Jul 2, 2025

A Protocol for the Production of KLRG1 Tetramer
07:24

A Protocol for the Production of KLRG1 Tetramer

Published on: January 12, 2010

10.0K

KLF4 and CD55 expression and function depend on each other.

Feng-Qi An1, Guangjin Zhou2, Micah T Harland1

  • 1Institute of Pathology Case Western Reserve University and Cardiovascular Research Institute, Department of Medicine, Harrington Heart and Vascular Institute, University Hospitals Cleveland Medical Center, Cleveland, OH, United States.

Frontiers in Immunology
|February 26, 2024
PubMed
Summary
This summary is machine-generated.

Kruppel-like factor 4 (KLF4) and CD55 work together to control inflammation and blood clotting. This study reveals a new gene activation mechanism where CD55 helps KLF4 regulate important proteins in endothelial cells and macrophages.

Keywords:
CBPCD55CD55 (DAF)CREBCREB binding protein (CBP)KLF4Kruppel like factor 4(KLF4) genedecay accelerating factor (CD55)

More Related Videos

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

6.8K
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.5K

Related Experiment Videos

Last Updated: Jul 2, 2025

A Protocol for the Production of KLRG1 Tetramer
07:24

A Protocol for the Production of KLRG1 Tetramer

Published on: January 12, 2010

10.0K
Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

6.8K
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.5K

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Kruppel-like factor 4 (KLF4) is crucial for maintaining homeostasis by regulating immunosuppressive and anti-thrombotic proteins.
  • The precise signals controlling KLF4 expression and its transactivation mechanism are not fully understood.
  • CD55 (decay accelerating factor) modulates immune responses and inflammation by regulating C3a and C5a receptor signaling.

Purpose of the Study:

  • To investigate the functional relationship between KLF4 and CD55.
  • To elucidate the mechanism by which CD55 influences KLF4-mediated gene regulation.
  • To explore the role of the KLF4-CD55 axis in endothelial cells (ECs) and macrophages.

Main Methods:

  • Analysis of CD55 and KLF4 expression in vascular endothelial cells (ECs) and macrophages.
  • Investigating the role of p-CREB and CREB Binding Protein (CBP) in KLF4-CD55 interactions.
  • Utilizing gene silencing techniques to assess the impact of CD55 on KLF4 function and expression.
  • Examining signaling pathways including adenylyl cyclase activation and C3a/C5a receptor signaling.

Main Results:

  • KLF4 expression upregulated CD55 in ECs via a p-CREB and CBP-dependent mechanism.
  • CD55 was essential for KLF4's regulation of pro-inflammatory and homeostatic proteins in ECs and macrophages.
  • Upregulated CD55 facilitated the recruitment of p-CREB and CBP to KLF4 for transcription.
  • Silencing CD55 impaired KLF4's ability to downregulate inflammatory proteins and reduced KLF4 expression itself.

Conclusions:

  • KLF4 and CD55 are linked through a novel gene transactivation mechanism involving p-CREB and CBP recruitment.
  • CD55 plays a critical role in KLF4's immunosuppressive and anti-thrombotic functions.
  • The KLF4-CD55 axis represents a potential therapeutic target for inflammatory and thrombotic diseases.