Zymolyase removes callose from germinating pollen and pollen tube walls

The impermeable callosic wall that surrounds germinating pollen grains and pollen tubes is removed by brief treatment with Zymolyase, an enzyme preparation that hydrolyzes linear glucose polymers with beta-1,3 linkages. Zymolyase treatment does not interfere with pollen tube growth or cylosis in the vegetative cell.