Release of catecholamines follows suckling or electrical stimulation of mammary nerve in lactating rats

The hypothesis that catecholamines may be released by mammary gland stimulation during lactation was tested by measuring, with an HPLC electrochemical method, plasma epinephrine (E) and norepinephrine (NE) concentrations during suckling in conscious rats and during electrical stimulation (pulses: 1 msec duration, 10/sec at 5-30 V) of the central end of a cut abdominal mammary nerve in urethane-anesthetized rats. Plasma E and NE concentrations were significantly elevated in two different strains of rats (Wistar and Holtzman) within 5 min of suckling. The concentration of E and NE did not change in control unsuckled rats during the same time period. As a complementary indication of sympathetic activation, it was observed that piloerection occurred during suckling. Plasma E levels (but not NE levels) increased significantly within 30 sec of a 2-min period of nerve stimulation in lactating rats on either day 7 or day 21 of lactation, as well as in nonlactating rats. The effect was significantly greater in nonlactating rats. The levels of E and NE were not altered after sham stimulation, whereas adrenalectomy abolished the rise in plasma E after mammary nerve stimulation. Blockade of the rise in plasma E also occurred after rapid injection of 100 microliters milk intraductally into each of two thoracic mammary glands, 15 sec before the onset of mammary nerve stimulation. These results show that E and NE can be released in response to suckling, and that activation of ductal mechanoreceptors may inhibit such release. These mechanisms may operate to regulate the rate of milk removal during suckling in the rat.