Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Promoting contraceptive use among unmarried female migrants in one factory in Shanghai: a pilot workplace intervention.

BMC health services research·2007
Same author

Apoptosis related protein 3, an ATRA-upregulated membrane protein arrests the cell cycle at G1/S phase by decreasing the expression of cyclin D1.

Biochemical and biophysical research communications·2007
Same author

Efficient one-pot synthesis of highly substituted pyridin-2(1H)-ones via the Vilsmeier-Haack reaction of 1-acetyl,1-carbamoyl cyclopropanes.

Organic letters·2007
Same author

Effects of Andrographitis Paniculata extracts on the expression of CD40 in endothelial cells.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban·2007
Same author

Theoretical and experimental investigations on the size of alginate microspheres prepared by dropping and spraying.

Journal of microencapsulation·2007
Same author

Beneficial effects of losartan on vascular injury induced by advanced glycosylation end products and their receptors in spontaneous hypertension rats.

Molecular and cellular biochemistry·2007
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Jul 2, 2025

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues
09:50

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues

Published on: February 9, 2024

1.3K

An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli.

Lin Bai1, Yaping Wu2, Wenshu Dai3

  • 1Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University; Core Facility of West China Hospital, Sichuan University.

Journal of Visualized Experiments : Jove
|February 26, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to visualize mouse kidney glomeruli using FITC-Dextran and CUBIC clearing. This technique allows for detailed 3D imaging and analysis of glomeruli number and volume.

More Related Videos

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K
Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
11:13

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Published on: April 17, 2013

10.5K

Related Experiment Videos

Last Updated: Jul 2, 2025

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues
09:50

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues

Published on: February 9, 2024

1.3K
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K
Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
11:13

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Published on: April 17, 2013

10.5K

Area of Science:

  • Nephrology
  • Biomedical Imaging
  • Histology

Background:

  • Glomeruli are vital for kidney function and pathology.
  • Current glomeruli observation methods are complex and may lose 3D details.
  • Tissue clearing technologies offer improved imaging depth and accuracy.

Purpose of the Study:

  • To establish a rapid and effective method for labeling and observing mouse kidney glomeruli.
  • To overcome limitations of existing glomeruli imaging techniques.
  • To enable detailed 3D analysis of glomeruli structure and distribution.

Main Methods:

  • FITC-Dextran (medium molecular weight) was injected intravenously into mice.
  • Kidneys were processed using the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) tissue clearing method.
  • Cleared kidneys were imaged using light-sheet microscopy to capture 3D glomeruli data.

Main Results:

  • Mouse glomeruli were rapidly and effectively labeled using FITC-Dextran and CUBIC clearing.
  • 3D image stacks of entire cleared mouse kidneys were obtained.
  • Software analysis allowed for digitization and measurement of glomeruli number, volume, and frequency.

Conclusions:

  • This method provides a significant advancement for studying kidney glomeruli in 3D.
  • The technique facilitates accurate and detailed quantitative analysis of glomeruli.
  • It offers a valuable tool for renal research, improving understanding of kidney function and disease.