LncRNA DGUOK-AS1 Promotes Cell Progression in Lung Squamous Cell Carcinoma by Regulation of miR-653-5p/SLC6A15 Axis
View abstract on PubMed
Summary
This summary is machine-generated.Long noncoding RNA DGUOK-AS1 promotes lung squamous cell carcinoma (LUSC) by upregulating SLC6A15. This study elucidates the mechanism involving DGUOK-AS1, miR-653-5p, and SLC6A15 in LUSC progression.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Long noncoding RNAs (lncRNAs) are crucial regulators in cancer development.
- LncRNA deoxyguanosine kinase antisense RNA 1 (DGUOK-AS1) has been implicated as a tumor promoter.
Purpose Of The Study
- To investigate the functional mechanism of DGUOK-AS1 in lung squamous cell carcinoma (LUSC).
- To explore the regulatory network involving DGUOK-AS1, miR-653-5p, and SLC6A15 in LUSC.
Main Methods
- Quantitative reverse transcription PCR (RT-qPCR) for gene expression analysis.
- Cell viability, proliferation, migration, and invasion assays (CCK-8, colony formation, Transwell).
- Luciferase reporter, RNA pulldown, and RNA immunoprecipitation assays to confirm molecular interactions.
Main Results
- DGUOK-AS1 was significantly upregulated in LUSC cells.
- Knockdown of DGUOK-AS1 inhibited LUSC cell proliferation, migration, and invasion.
- DGUOK-AS1 directly interacted with miR-653-5p, which targets SLC6A15, thereby modulating SLC6A15 expression in LUSC cells.
- Overexpression of SLC6A15 rescued the inhibitory effects of DGUOK-AS1 knockdown.
Conclusions
- DGUOK-AS1 promotes malignant behaviors in LUSC by upregulating SLC6A15.
- The DGUOK-AS1/miR-653-5p/SLC6A15 axis represents a potential therapeutic target for LUSC.
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