Comparison of sediment biomarker signatures generated using time-integrated and discrete suspended sediment samples

Sediment source fingerprinting using biomarker properties has led to new insights in our understanding of land use contributions to time-integrated suspended sediment samples at catchment scale. A time-integrated mass-flux sampler (TIMS; also known as the 'Phillips' sampler), a cost-effective approach for suspended sediment collection in situ. Such samplers are widely being used to collect sediment samples for source fingerprinting purposes, including studies using biomarkers as opposed to more conventional tracer properties. Here, we assessed the performance of TIMS for collecting representative sediment samples for biomarkers during high discharge events in a small lowland grassland-dominated catchment. Concentrations of long odd-chain n-alkanes (> C₂₃) and both saturated free and bound fatty acids (C₁₄-C₃₂), as well as compound-specific ¹³C were compared between sediment collected by both TIMS and autosamplers (ISCO). The results showed that concentrations of alkanes, free fatty acids, and bound fatty acids are consistently comparable between TIMS and ISCO suspended sediment samples. Similarly, compound-specific ¹³C signals were not found to be significantly different in the suspended sediment samples collected using the different samplers. However, different magnitudes of resemblance in biomarker concentrations and compositions between the samples collected using the two sediment collection methods were confirmed by overlapping index and symmetric coordinates-based correlation analysis. Here, the difference is attributed to the contrasting temporal basis of TIMS (time-integrated) vs. ISCO (discrete) samples, as well as potential differences in the particle sizes collected by these different sediment sampling methods. Nevertheless, our findings suggest that TIMS can be used to generate representative biomarker data for suspended sediment samples collected during high discharge events.