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Ameliorative effects of thymoquinone on the caspase 3, kidney function and oxidative stress tartrazine-induced

Zeynep Erdemli1, Mehmet Gul2, Nurcan Gokturk1

  • 1Department of Medical Biochemistry, Medical Faculty, Inonu University, Malatya, Turkey.

Toxicon : Official Journal of the International Society on Toxinology
|February 26, 2024
PubMed

Insights

This study reveals that the food dye Tartrazine causes kidney damage by increasing oxidative stress. However, Thymoquinone effectively protects against this Tartrazine-induced nephrotoxicity due to its potent antioxidant properties.

Area of Science:

  • Toxicology
  • Pharmacology
  • Biochemistry

Background:

  • Tartrazine is a widely used synthetic food dye with potential adverse health effects.
  • Nephrotoxicity is a significant concern associated with exposure to certain industrial chemicals.
  • Thymoquinone, derived from Nigella sativa, is recognized for its antioxidant and anti-inflammatory properties.

Purpose of the Study:

  • To investigate the impact of Tartrazine on kidney function and structure.
  • To evaluate the protective efficacy of Thymoquinone against Tartrazine-induced nephrotoxicity in a rat model.

Main Methods:

  • Wistar albino rats were divided into four groups: control, Tartrazine, Thymoquinone, and Tartrazine + Thymoquinone.
  • Animals were treated for three weeks, followed by the collection of kidney tissues and blood samples.
  • Biochemical markers of oxidative stress (MDA, SOD, TOS, OSI, GSH, GSH-Px, CAT, TAS) and kidney function (BUN, Creatinine) were analyzed.
  • Histopathological examination of kidney tissues was performed to assess structural damage.

Main Results:

  • Tartrazine administration led to increased oxidative stress markers and elevated BUN and Creatinine levels, indicating kidney dysfunction.
  • Histopathology revealed glomerular and tubular damage, along with inflammatory infiltration in the kidneys of the Tartrazine group.
  • Co-administration of Thymoquinone with Tartrazine significantly improved biochemical parameters and ameliorated histopathological damage.
  • Thymoquinone treatment alone did not show adverse effects and maintained normal kidney function and structure.

Conclusions:

  • Tartrazine induces significant nephrotoxicity, characterized by oxidative stress and impaired kidney function.
  • Thymoquinone exhibits potent antioxidant and protective effects against Tartrazine-induced kidney damage.
  • Thymoquinone holds promise as a therapeutic agent to mitigate Tartrazine-induced toxicity.
Keywords:
Caspase 3NephrotoxicityOxidative stressTartrazineThymoquinone

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