Bacterial Patterning: A Promising Biofabrication Technique

Area of Science:

• Biomedical Engineering
• Synthetic Biology
• Microbiology

Background:

• Bacterial patterning is a crucial biofabrication technique in biomedicine.
• Diverse bacterial patterning approaches have been developed over the last 20 years.
• A comprehensive review of these technologies is currently lacking.

Purpose of the Study:

• To systematically summarize the advancements in bacterial patterning over the past two decades.
• To provide an overview of bacterial patterning strategies, including their principles, advantages, and limitations.
• To highlight the biomedical applications of bacterial patterning, such as biofilm control, biosensing, and biointervention.

Main Methods:

• Systematic review of literature on bacterial patterning published in the last 20 years.
• Elucidation of the definition and fundamental principles of bacterial patterning.
• Categorization and discussion of established bacterial patterning strategies.
• Showcasing of biomedical applications with efficacy examples.

Main Results:

• Detailed summary of bacterial patterning progress over the last two decades.
• Analysis of various bacterial patterning strategies, outlining their respective pros and cons.
• Demonstration of successful applications in spatial control of biofilms, biosensing, and biointervention.
• Identification of key challenges and future opportunities in the field.

Conclusions:

• Bacterial patterning has significantly evolved, offering precise spatial control for biomedical applications.
• This mini-review serves as a concise guide to the field's progress, methods, and applications.
• Future research should focus on overcoming existing challenges and exploring new opportunities in bacterial patterning.