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Updated: Jul 2, 2025

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

Cellular transfection using rapid decrease in hydrostatic pressure.

Shudi Huang¹, Nan Ji Suo², Tyler R Henderson³

¹Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, M5S 3M2, Canada.

Scientific Reports

|February 26, 2024

Summary

This summary is machine-generated.

Pressure-jump-poration (PJP) offers a novel, safe, and cost-effective method for introducing nucleic acids into cells, including challenging primary cell types like embryonic stem cells. This technique enhances genetic modification efficiency across various research and clinical applications.

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Last Updated: Jul 2, 2025

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Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Nucleic acid delivery is fundamental to molecular biology.
Existing transfection methods have limitations in cost, safety, efficacy, and applicability to difficult-to-transfect cells.
An ideal transfection method requires high efficiency, safety, scalability, and broad applicability.

Purpose of the Study:

To introduce a novel transfection method, Pressure-jump-poration (PJP).
To evaluate PJP's efficacy, safety, and applicability, particularly for challenging cell types.
To explore potential insights into cellular transformation by comparing PJP in primary vs. transformed cells.

Main Methods:

Developed and utilized Pressure-jump-poration (PJP), a technique employing rapid depressurization for cell transfection.
Applied PJP to introduce various genetic modifiers into cells.
Compared transfection efficiency and outcomes in primary and transformed cell types.

Main Results:

PJP successfully transfected difficult-to-modify primary cells, including embryonic stem cells.
The method demonstrated safe and sterile introduction of genetic modifiers.
A notable dichotomy was observed in PJP-induced transfection between primary and transformed cells.

Conclusions:

Pressure-jump-poration (PJP) presents a promising new method for efficient and safe nucleic acid delivery.
PJP overcomes limitations of existing methods, especially for primary and hard-to-transfect cells.
The observed differences in transfection between cell types may offer new perspectives on cellular transformation.