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A Comparative Phenotypic and Genomic Analysis of Methicillin-Resistant Staphylococcus aureus ST45 Isolates From

Kuo-Ti Peng1,2, Pei-Chun Chen1, Jiun-Liang Chen1

  • 1Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Chiayi.

The Journal of Infectious Diseases
|February 26, 2024
PubMed
Abstract

Insights

Methicillin-resistant Staphylococcus aureus (MRSA) ST45 isolates from osteomyelitis are more pathogenic and drug-resistant than those from cellulitis. These findings highlight the public health threat posed by multidrug-resistant MRSA ST45 strains.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 45 is a globally prevalent lineage.
  • Distinctive characteristics of MRSA ST45 isolates from cellulitis versus osteomyelitis were unexplored.

Purpose of the Study:

  • To investigate phenotypic and genomic differences between MRSA ST45 isolates from cellulitis and osteomyelitis.
  • To assess antimicrobial susceptibility, biofilm formation, cellular infectivity, and in vivo pathogenicity.

Main Methods:

  • Collected 15 MRSA ST45 isolates (6 cellulitis, 9 osteomyelitis) from Taiwan.
  • Characterized isolates for antimicrobial resistance, biofilm formation, and infectivity.
  • Performed whole-genome sequencing (WGS) on selected isolates.

Main Results:

  • Osteomyelitis MRSA ST45 isolates exhibited resistance to ciprofloxacin, levofloxacin, gentamicin, and doxycycline, unlike cellulitis isolates.
  • Osteomyelitis isolates showed enhanced biofilm formation, cellular infectivity, and greater virulence in mice.
  • WGS identified mutations in virulence/resistance genes and unique transposable elements (IS256, IS257R2) in osteomyelitis isolates.

Conclusions:

  • MRSA ST45 isolates from osteomyelitis are highly pathogenic and multidrug-resistant.
  • These findings indicate a significant public health risk associated with these specific MRSA strains.
Keywords:
MRSAST45cellulitisosteomyelitiswhole-genome sequencing

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