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Updated: Jul 2, 2025

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
Published on: April 6, 2022
Visible-light-driven photo-peroxidase catalysis: high-efficiency degradation of indole in water
Chongrui Xu1, Ping Xue1, Rui Li1
1State Key Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering, College of Chemistry & Chemical Engineering, Ningxia University Yinchuan 750021 China ping@nxu.edu.cn.
Abstract:
The demand for H2O2 restricts the wider application of horseradish peroxidase (HRP) in degradation. In this work, a novel photoenzyme synergistic catalytic system was developed for high-efficiency degrading of indole in water by HRP without extra H2O2. The HRP was immobilized on CN-ZIF prepared by the combination of g-C3N4 and ZIF-8 to achieve photo-peroxidase catalyst HRP/Zn-CN-ZIF. Under visible light, photogenerated electrons and H2O2 from HRP/Zn-CN-ZIF participated in the biocatalytic cycle of HRP directly. As a result, the indole at 20 mg L-1 in water was degraded completely in 2 h by the HRP/Zn-CN-ZIF photoenzyme synergistic catalytic system without the addition of H2O2. Furthermore, HRP/Zn-CN-ZIF exhibited superior visible light absorption and charge transfer ability compared to g-C3N4. The results of the mechanism studies suggest that ·OH would play the most significant role from the HRP/Zn-CN-ZIF in indole degradation. This research provides an efficient approach for the removal of indole from water environments.
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