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Effects of Preanalytical Sample Collection and Handling on Comprehensive Metabolite Measurements in Human Urine
John Braisted1, Theresa Henderson2, John W Newman3,4
1Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville, MD.
Urine metabolomic profiles are affected by sample handling, with borate preservatives causing the most significant changes. Consistent handling is recommended to ensure reliable results in epidemiology studies.
Area of Science:
- Metabolomics
- Clinical Chemistry
- Epidemiology
Background:
- Epidemiology studies link metabolomic profiles to disease risk.
- Urine is a preferred biospecimen for non-invasive collection.
- Understanding preanalytical variability is crucial for robust urinary metabolomic studies.
Conclusions:
- Preanalytical sample handling conditions demonstrably alter urinary metabolomic profiles.
- Borate preservatives have the most substantial impact on measured metabolites.
- While generally smaller than serum, consistent urine sample handling and robustness evaluation are essential for reliable epidemiological findings.
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