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hsa_circ_0087100/hsa-miR-6743-5p affects Th1 cell differentiation by regulating STAT1 in diabetic retinopathy

Shuai He1,2, Dongwei Lai1,2, Chenggong Ma3

  • 1Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.

Epigenomics
|February 27, 2024
PubMed

Insights

The competitive endogenous RNA (ceRNA) network, specifically hsa_circ_0087100/hsa-miR-6743-5p/STAT1, influences Th1 cell differentiation in diabetic retinopathy (DR). This finding clarifies the role of ceRNA in DR immune infiltration.

Area of Science:

  • Immunology
  • Genetics
  • Ophthalmology

Background:

  • Diabetic retinopathy (DR) involves complex immune dysregulation.
  • The role of competitive endogenous RNA (ceRNA) networks in DR-associated immune infiltration remains unclear.

Purpose of the Study:

  • To investigate the function of the ceRNA network in immune cell infiltration within diabetic retinopathy.
  • To identify specific ceRNA pathways contributing to Th1 cell differentiation in DR.

Main Methods:

  • Utilized Gene Expression Omnibus data for differentially expressed circRNAs, miRNAs, and mRNAs.
  • Employed CIBERSORT and ssGSEA for immune infiltration analysis.
  • Conducted weighted gene co-expression network analysis to identify co-expressed genes.
  • Validated STAT1-mediated Th1 differentiation in vitro and in vivo DR models.

Main Results:

  • Identified the hsa_circ_0087100/hsa-miR-6743-5p/STAT1 axis as a key component in Th1 cell immune infiltration.
  • Confirmed aberrant expression of this ceRNA network and STAT1-mediated Th1 differentiation in DR.
  • Demonstrated the involvement of this pathway in both cellular and animal models of DR.

Conclusions:

  • The hsa_circ_0087100/hsa-miR-6743-5p/STAT1 ceRNA network plays a significant role in modulating Th1 cell differentiation in diabetic retinopathy.
  • This study provides novel insights into the molecular mechanisms underlying immune responses in DR.

Keywords:
STAT1Th1 cellceRNA networkdiabetic retinopathyimmune regulation

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