Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Endoplasmic Reticulum

The Endoplasmic Reticulum

The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...

Endoplasmic Reticulum

Endoplasmic Reticulum

The Endoplasmic Reticulum (ER) in eukaryotic cells is a substantial network of interconnected membranes with diverse functions, from calcium storage to biomolecule synthesis. A primary component of the endomembrane system, the ER manufactures phospholipids critical for membrane function throughout the cell. Additionally, the two distinct regions of the ER specialize in the manufacture of specific lipids and proteins.

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An ER-microtubule bridge: Reticulon 17 links microtubules with ER network organisation in plants.

The Plant journal : for cell and molecular biology·2026

Same author

The risk of distant organ metastasis and survival analysis in esophageal cancer with different histological subtypes.

Esophagus : official journal of the Japan Esophageal Society·2026

Same author

Ubiquitin-dependent mitochondrial protein degradation ensures seedling emergence by regulating ER-mitochondrial interaction and mitophagy.

Nature plants·2026

Same author

COST1 stimulates RHD3 GTPase activity to maintain ER morphology and plant growth in <i>Arabidopsis</i>.

Science advances·2026

Same author

Advances in research on the role of VEGF family members in ischemic stroke.

Frontiers in neuroendocrinology·2026

Same author

Autophagy is more vital in tomato and citrus than in arabidopsis.

Protoplasma·2026

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 2, 2025

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Make It Shine: Labelling the ER for Light and Fluorescence Microscopy.

Chris Hawes¹, Pengwei Wang², Verena Kriechbaumer³

¹Endomembrane Structure and Function Research Group, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)

|February 27, 2024

Summary

This summary is machine-generated.

This study presents new methods for visualizing the plant endoplasmic reticulum (ER) in its natural state. These techniques use fluorescent proteins, lipid dyes, and immunolabelling for detailed imaging of this dynamic organelle.

Keywords:

Endoplasmic reticulum Fluorescent dyes Fluorescent protein Immunofluorescence Labelling Stable Transient expression

More Related Videos

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Published on: December 10, 2016

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

Related Experiment Videos

Last Updated: Jul 2, 2025

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Published on: December 10, 2016

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

Area of Science:

Plant cell biology
Organelle imaging
Molecular biology

Background:

The endoplasmic reticulum (ER) is a crucial organelle within plant cells, forming a dynamic network of tubules and cisternae.
Understanding the ER's structure and function is vital for comprehending overall plant cell physiology.
Visualizing the ER in its native, mobile state presents significant technical challenges.

Purpose of the Study:

To develop and describe effective methods for labeling and imaging the native plant endoplasmic reticulum (ER).
To provide researchers with tools for observing the ER's dynamic behavior in planta.
To facilitate detailed studies of ER structure and function in living plant cells.

Main Methods:

Utilizing fluorescent proteins for in vivo ER labeling.
Employing lipid dyes to visualize ER membranes.
Implementing immunolabelling techniques on plant tissues for ER visualization.

Main Results:

Successful labeling of the native plant ER using fluorescent proteins and lipid dyes.
Demonstration of effective immunolabelling protocols for ER detection in plant tissues.
Acquisition of high-resolution images capturing the dynamic nature of the ER.

Conclusions:

The described methods enable robust imaging of the plant ER in its native state.
These techniques are valuable for advancing research in plant cell biology and organelle dynamics.
The study provides a foundation for further investigations into ER function and interactions within plant cells.