Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Microtubule-associated protein 70 links brassinosteroid signaling to fruit morphogenesis in tomato.

Journal of plant physiology·2026

Same author

The risk of distant organ metastasis and survival analysis in esophageal cancer with different histological subtypes.

Esophagus : official journal of the Japan Esophageal Society·2026

Same author

Ubiquitin-dependent mitochondrial protein degradation ensures seedling emergence by regulating ER-mitochondrial interaction and mitophagy.

Nature plants·2026

Same author

COST1 stimulates RHD3 GTPase activity to maintain ER morphology and plant growth in <i>Arabidopsis</i>.

Science advances·2026

Same author

Advances in research on the role of VEGF family members in ischemic stroke.

Frontiers in neuroendocrinology·2026

Same author

Autophagy is more vital in tomato and citrus than in arabidopsis.

Protoplasma·2026

Search research articles

Related Experiment Video

Updated: Jul 2, 2025

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Published on: October 20, 2023

Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy.

Lifan Li^1,2, Tong Zhang^1,2, Patrick J Hussey³

¹National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Methods in Molecular Biology (Clifton, N.J.)

|February 27, 2024

Summary

Researchers developed new methods to study plant endoplasmic reticulum-plasma membrane contact sites (EPCS). These techniques help identify proteins at EPCS, aiding in understanding their cellular functions and the structure of these vital membrane connections.

Keywords:

Chi-square analysis ER-PM contact sites Endoplasmic reticulum Immunogold TEM N. benthamiana VAP27 family

More Related Videos

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Published on: December 17, 2012

Related Experiment Videos

Last Updated: Jul 2, 2025

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Published on: October 20, 2023

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Published on: December 17, 2012

Area of Science:

Plant cell biology
Membrane biophysics
Subcellular structure

Background:

The plant endoplasmic reticulum (ER) network connects to the plasma membrane (PM), forming specialized structures called ER-PM contact sites (EPCS).
EPCS are crucial for cellular processes and are maintained by a complex of ER integral membrane proteins, PM-associated proteins, and cytoskeletal elements.

Purpose of the Study:

To describe novel methodologies for investigating the structural organization of plant EPCS.
To identify and characterize proteins associated with EPCS, facilitating the prediction of their subcellular functions.

Main Methods:

Utilizing artificially constructed reporter systems to visualize EPCS dynamics.
Employing GFP-tagged protein expression and advanced image analysis techniques.
Applying immunogold labeling for ultrastructural localization of EPCS-associated proteins.

Main Results:

The described methods enable detailed structural analysis of EPCS.
Successful identification of potential EPCS-associated proteins and their precise localization.
Validation of techniques for studying protein complexes at membrane contact sites.

Conclusions:

The developed methods provide powerful tools for dissecting the molecular architecture of plant EPCS.
Identifying EPCS proteins aids in understanding their roles in cellular signaling and membrane trafficking.
This research advances the study of eukaryotic membrane contact site biology.