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Organ-Specific Microsomes from Dark-Grown Hypocotyls of Arabidopsis thaliana
Seinab Noura1,2, Jürgen Kleine-Vehn3,4, Sascha Waidmann3
1Institute of Biology II, Chair group of Molecular Plant Physiology (MoPP), University of Freiburg, Freiburg, Germany. seinab.noura@biologie.uni-freiburg.de.
Abstract:
In this book chapter, we present a method for microsome isolation from the hypocotyl tissue of dark-grown Arabidopsis thaliana. Microsomes are heterogeneous, vesicle-like membranes, which are, not exclusively, derived but enriched with membranes of the endoplasmic reticulum (ER). Here, we describe the experimental setup, including sample preparation, homogenization, differential centrifugation steps, and quality control measures after microsome isolation.
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