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Sterilization of Human Amniotic Membrane Using an Ozone Hydrodynamic System
Túlia Botelho1,2, Bianca Akemi Kawata3,4, Silvia Móbille Awoyama1,5
1Center for Innovation, Technology and Education - CITÉ, Parque de Inovação Tecnológica de São José dos Campos, São José dos Campos, SP, 12247-016, Brazil.
Annals of Biomedical Engineering
|February 27, 2024
Summary
Ozonated dynamic water effectively sterilizes human amniotic membrane (hAM) against common microbes within 15 minutes, offering a promising alternative to gamma-irradiation for tissue engineering scaffolds. This method also shows potential for de-epithelialization.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Microbiology
Background:
- Human amniotic membrane (hAM) is a valuable biomaterial for tissue engineering due to its regenerative properties.
- Conventional sterilization methods like gamma-irradiation can damage hAM structure and growth factors.
- There is a need for alternative sterilization techniques that preserve hAM integrity.
Purpose of the Study:
- To evaluate the efficacy of ozonated dynamic water for sterilizing human amniotic membrane (hAM).
- To assess the impact of ozonation time on microbial inactivation and depyrogenation.
- To investigate the effect of ozone sterilization on the structural integrity of hAM.
Main Methods:
- hAM fragments were experimentally contaminated with Staphylococcus aureus, Escherichia coli, Candida albicans, Staphylococcus epidermidis, and Clostridium sporogenes.
- Contaminated hAM samples were subjected to sterilization using ozonated dynamic water for varying durations (5-15 minutes).
- Histological analysis was performed to evaluate structural changes in the hAM post-ozonation. Depyrogenation was assessed at longer ozonation times (15-35 minutes).
Main Results:
- Sterilization was achieved for Staphylococcus aureus and Clostridium sporogenes within 10 minutes.
- Escherichia coli and Staphylococcus epidermidis were sterilized within 15 minutes.
- Candida albicans microbial activity decreased with exposure time but was not fully eliminated within the tested timeframe. Promising depyrogenation results were observed after 35 minutes of ozonation.
- Histological analysis revealed a decrease in epithelium thickness and some damage with increased ozone exposure, suggesting potential for de-epithelization.
Conclusions:
- Ozonated dynamic water is an effective sterilization method for hAM against several common bacteria.
- Ozone treatment shows potential for depyrogenation of hAM.
- Ozone exposure can lead to hAM de-epithelization, making it a potential method for preparing scaffolds for tissue engineering.
Keywords:
Biomaterials sterilizationCellular scaffoldDe-epithelizationHuman amniotic membraneOzoneTissue engineeringMore Related Videos
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