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Related Concept Videos

Calmodulin-dependent Signaling01:16

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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
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The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
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Polyaspartic Acid-Calcium-Lanthanum Complexes Induce Antibacterial Remineralization of Dentin and In-Depth Occlusion of Dentinal Tubules.

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In-depth Occlusion of Dentinal Tubules Induced by Polyaspartic Acid-Calcium and Magnesium Complexes.

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STMP and PVPA as Templating Analogs of Noncollagenous Proteins Induce Intrafibrillar Mineralization of Type I Collagen via PCCP Process.

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Citrate Improves Biomimetic Mineralization Induced by Polyelectrolyte-Cation Complexes Using PAsp-Ca&Mg Complexes.

Dong-Ni Shen1, Yue-Dan Xu1, Cheng He2

  • 1Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou, Zhejiang, 310000, China.

Advanced Healthcare Materials
|February 27, 2024
PubMed
Summary

Citrate enables magnesium-doped hydroxyapatite mineralization in collagen fibrils, overcoming magnesium

Keywords:
citratecollagen mineralizationdentinmagnesiumpolyelectrolyte–cation complex

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Area of Science:

  • Biomaterials Science
  • Biomineralization
  • Materials Chemistry

Background:

  • Magnesium ions are crucial in hard tissue mineralization but paradoxically inhibit hydroxyapatite (HAp) crystallization.
  • The mechanism regulating magnesium-doped biomimetic mineralization within collagen fibrils remains unclear.

Purpose of the Study:

  • To elucidate the mechanism by which citrate regulates magnesium-doped biomimetic mineralization of collagen fibrils.
  • To investigate the potential of citrate-mediated mineralization for creating antibacterial biomaterials.

Main Methods:

  • Utilizing citrate to modify collagen fibrils and influence the assembly of polyaspartic acid-Ca&Mg complexes and phosphate.
  • Investigating the role of citrate in overcoming magnesium ion-induced inhibition of HAp crystallization.
  • Assessing the antibacterial properties and biocompatibility of the resulting magnesium-doped HAp biomaterials.

Main Results:

  • Citrate enhances collagen fibril electronegativity, facilitating the attraction of mineral precursors.
  • Citrate disrupts the hydration layer of magnesium ions, mitigating their inhibitory effect on HAp crystallization.
  • The remineralized dentin exhibits antibacterial properties, and the mineralization medium shows excellent biocompatibility.

Conclusions:

  • Citrate plays a key role in regulating magnesium-doped biomimetic mineralization by modifying collagen fibrils and overcoming inhibitory ions.
  • This strategy offers a novel approach for developing antibacterial biomaterials through controlled biomineralization.
  • Highlights the significant, yet often overlooked, regulatory role of small biomolecules like citrate in biomineralization processes.