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Multiple sclerosis: activated cells in cerebrospinal fluid in acute exacerbations
Annals of Neurology
|December 1, 1985
Summary
Cerebrospinal fluid (CSF) lymphocytes from multiple sclerosis patients show activation during acute exacerbations. Activated cells predominantly express the OKT4 phenotype, indicating a specific immune response in the central nervous system.
Area of Science:
- Neuroimmunology
- Cellular immunology
Background:
- Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system.
- Acute exacerbations involve active immune processes within the CNS.
Purpose of the Study:
- To investigate the activation status and phenotype of cerebrospinal fluid (CSF) lymphocytes during acute multiple sclerosis exacerbations.
- To utilize a novel flow cytometry method for simultaneous cell-surface antigen and cell-cycle analysis.
Main Methods:
- Flow cytometry analysis of CSF lymphocytes from three patients with active MS.
- Simultaneous measurement of cell-surface antigens and cell-cycle phase.
Main Results:
- CSF lymphocytes from patients in acute MS exacerbations were found to be activated.
- The majority of activated lymphocytes exhibited the OKT4 phenotype.
- A minimal number of activated cells expressed the OKT8 phenotype.
Conclusions:
- CSF lymphocytes are activated during acute multiple sclerosis exacerbations.
- The OKT4+ T-cell subset appears to be predominantly involved in the immune response during these events.