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DEPArray™ single-cell technology: A validation study for forensic applications.

Janine Schulte1, Amke Caliebe2, Michael Marciano3

  • 1Institute of Forensic Medicine, University Basel, Pestalozzistrasse 22, Basel 4056, Switzerland.

Forensic Science International. Genetics
|February 27, 2024
PubMed
Summary
This summary is machine-generated.

The DEPArray™ technology effectively deconvolutes biological mixtures for forensic DNA analysis. This validation study confirms its reliability for single-cell separation and genotyping, crucial for complex casework like cold cases.

Keywords:
DEPArray™ technologyDNA mixture deconvolutionImage-based digital cell sortingSingle-cell genotypingValidation

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Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Forensic investigations frequently involve complex biological mixtures from multiple individuals.
  • Accurate deconvolution of these mixtures is essential for reliable DNA profiling and casework.
  • DEPArray™ technology offers a promising solution for separating individual cell populations prior to genetic analysis.

Purpose of the Study:

  • To systematically validate the DEPArray™ system for specificity, sensitivity, repeatability, and contamination in forensic samples.
  • To evaluate the DEPArray™ system's performance for single-cell analysis of blood, epithelial, and sperm cells.
  • To assess the potential of DEPArray™ for complex forensic applications, including cold cases and mixed offender profiles.

Main Methods:

  • Validation of DEPArray™ technology using blood, epithelial, and sperm cells.
  • Genetic analysis of single cells using the NGM Detect™ Amplification Kit.
  • Application of a consensus approach for profile generation, mimicking real casework scenarios.

Main Results:

  • DEPArray™ successfully identified cell types and assigned genotypes to single cells.
  • Diploid cell analysis achieved ~80% completeness; haploid sperm analysis yielded ~51% completeness.
  • Complete profiles were obtained from as few as 3 diploid cells or 15 sperm cells, with rare contamination events.

Conclusions:

  • The DEPArray™ system is a validated and reliable technology for single-cell separation and forensic DNA analysis of complex mixtures.
  • The consensus approach enhances profile generation, particularly for challenging samples like sperm DNA.
  • DEPArray™ technology provides valuable guidance and potential solutions for difficult forensic casework.