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Related Experiment Videos

An epithelial scatter factor released by embryo fibroblasts.

M Stoker, M Perryman

    Journal of Cell Science
    |August 1, 1985
    PubMed
    Summary

    Human embryo fibroblast-conditioned medium contains a protein factor that causes epithelial cells to separate and scatter. This scattering factor affects cell migration and shape, with potential roles in epithelial cell mobilization.

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    Area of Science:

    • Cell Biology
    • Biochemistry

    Background:

    • Epithelial cells form crucial structural barriers.
    • Cell-cell junctions maintain tissue integrity.
    • Factors influencing epithelial cell behavior are key to understanding tissue dynamics.

    Purpose of the Study:

    • To investigate the effects of conditioned medium from human embryo fibroblasts on epithelial cell structure and behavior.
    • To characterize the nature of the active component responsible for cell scattering.

    Main Methods:

    • Utilized Madin-Darby Canine Kidney (MDCK) cells for an in vitro scattering assay.
    • Assessed activity through serial dilutions of conditioned medium.
    • Determined properties of the active factor including heat lability, non-dialyzability, and sensitivity to trypsin.

    Main Results:

    • Conditioned medium induced separation and scattering of epithelial cells by disrupting structural junctions.
    • The scattering activity was potent, effective at high dilutions (1:64) and low protein concentrations (<100 ng).
    • Cell separation initiated within 15 minutes and completed within 10 hours, also affecting MDCK cell migration and dome formation.

    Conclusions:

    • A heat-labile, trypsin-sensitive protein factor in human embryo fibroblast-conditioned medium disrupts epithelial cell junctions.
    • This factor promotes cell scattering and migration, suggesting a role in epithelial cell mobilization.
    • Further purification and identification of the factor are needed to elucidate its precise physiological function.

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